Dissecting the localization of lipopolysaccharide-responsive and beige-like anchor protein (LRBA) in the endomembrane system
Allergol. immunopatol
; 48(1): 8-17, ene.-feb. 2020. ilus
Artigo
em Inglês
| IBECS
| ID: ibc-186586
Biblioteca responsável:
ES1.1
Localização: BNCS
ABSTRACT
Introduction and objectives:
LRBA deficiency is caused by loss of LRBA protein expression, due to either homozygous or compounds heterozygous mutations in LRBA. LRBA deficiency has been shown to affect vesicular trafficking and autophagy. To date, LRBA has been observed in the cytosol, Golgi apparatus and some lysosomes in LPS-stimulated murine macrophages. The objectives of the present study were to study the LRBA localization in organelles involved in vesicular traffic, phagocytosis, and autophagy in mononuclear phagocytes (MP). Materials andmethods:
We analyzed LRBA colocalization with different endosomes markets using confocal microscopy in MP. We used the autophagy inhibitors to determine the role of LRBA in formation, maturation or degradation of the autophagosome.Results:
LRBA intracellular trafficking depends on the activity of the GTPase ADP ribosylation factor-1 (ARF) in MP. LRBA was identified in early, late endosomes but did not colocalize strongly with lysosomal markers. Although LRBA appears not to be recruited during the phagocytic cargo uptake, it greatly colocalized with the microtubule-associated protein 1A/1B-light chain 3 (LC3) under a steady state and this decreased after the induction of autophagy flux. Although the use of inhibitors of lysosome fusion did not restore the LRBA/LC3 colocalization, inhibitors of either early to late endosomes trafficking or PI3K pathway did.Conclusions:
Taken together, our results show that LRBA is located in endomembrane system vesicles, mainly in the early and late endosomes. Although LRBA appears not to be involved in the phagocytic uptake, it is recruited in the early steps of the autophagy fluxRESUMEN
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Coleções:
Bases de dados nacionais
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Espanha
Base de dados:
IBECS
Assunto principal:
Diferenciação Celular
/
Membrana Celular
/
Lipopolissacarídeos
/
Proteínas Adaptadoras de Transdução de Sinal
/
Síndromes de Imunodeficiência
Idioma:
Inglês
Revista:
Allergol. immunopatol
Ano de publicação:
2020
Tipo de documento:
Artigo
Instituição/País de afiliação:
Universidad de Antioquia UdeA/Colombia