Aqueous peel extract of Citrus aurantifolia attenuated tramadol-mediated testicular oxidative stress, apoptosis, inflammations and histo-architectural degeneration in a rat model

ABSTRACT

Tramadol is one of the most commonly abused drugs. It has been reported to impair testicular function in models. Citrus aurantifolia is a citrus fruit used extensively in beverages and herbal preparations. This study investigated the modulating role of aqueous zest extract of Citrus aurantifolia (AZECA) against tramadol-mediated testicular toxicity in rat. Twenty-five male rats were assigned to 5 groups and orally treated with distilled water (negative control) for 28 days, 30 mg/kg of tramadol (positive control) for 14 days, 30 mg/kg of tramadol for first two weeks and 50 (treatment 1) and 100 mg/kg (treatment 1) of AZECA for the last 2 weeks. Biochemical markers were evaluated and testes were stained routinely for morphometry. Results shows a significant (p<0.05) decrease in testosterone, FSH and LH, SOD, CAT and a significant increase MDA, IL-1 and TNF-alpha evidenced by a significant (p < 0.05) reduction in volumetric proportion of interstitium and seminiferous tubules-tunica propria, macrophages, blood vessels and lymphatic space, macrophages, connective tissue, diameter, cross-sectional area and number of pro-files of seminiferous tubules per unit area in group treated with tramadolalone. These parameters were significantly (p<0.05) negated in groups that had AZECA supplementation. This study has demonstrated that treatment with AZECA exerted a potent testiculo-protective activity against tramadol-induced testicular injury in rats

RESUMEN

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