Detection of SARS-CoV-2 using real-time polymerase chain reaction in different clinical specimens: A critical review
Allergol. immunopatol
; 49(1): 159-164, ene.-feb. 2021. tab
Artigo
em Inglês
| IBECS
| ID: ibc-197113
Biblioteca responsável:
ES1.1
Localização: BNCS
ABSTRACT
Coronavirus disease 2019 (COVID-19) is a disease caused by a new strain of coronavirus named as severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). Globally, since the outbreak, more than seven million confirmed cases of COVID-19 have been reported. The rapid spread and increase in the number of new cases is due to person-to-person transmission. To further control its transmission, early laboratory diagnosis of both asymptomatic and symptomatic patients is crucial. Presently, the COVID-19 diagnosis of infected individuals is dependent on computed tomography scanning and real-time polymerase chain reaction (PCR). The latter is considered more sensitive and efficient for early diagnosis. In this review, general comparisons are made (cases, fatality rate, incubation period, clinical features, and reservoirs) and diagnostic laboratory procedures (specimens, extraction methods, and positive rates by real-time PCR) are compared between SARS, Middle East Respiratory Syndrome, and SARS-2. In total, 8982 SARS-2 suspected patients specimen data were retrieved, in which 40.9% (n = 3678) were detected as positive by real-time PCR. The specimen-wise high detection rate was observed from bronchoalveolar lavage, followed by saliva, nasal swabs, and sputum. As the COVID-19 cases are persistently increasing, the selection of appropriate specimens and laboratory assay would help in rapid and timely diagnosis
RESUMEN
No disponible
Texto completo:
Disponível
Coleções:
Bases de dados nacionais
/
Espanha
Base de dados:
IBECS
Assunto principal:
Pneumonia Viral
/
Infecções por Coronavirus
/
Pandemias
/
Reação em Cadeia da Polimerase em Tempo Real
/
Betacoronavirus
Limite:
Humanos
Idioma:
Inglês
Revista:
Allergol. immunopatol
Ano de publicação:
2021
Tipo de documento:
Artigo
Instituição/País de afiliação:
Bacha Khan Medical College/Pakistan
/
Rehman medical Institute/Pakistan
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The University of Haripur/Pakistan