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Overexpression of ALDH1 and EMT marker profile are linked with unfavorable outcome in head and neck cancer
Vieira, Rúbia Rocha; Hildebrand, Laura Campos; Jesus, Luciano Henrique; Klabunde, Caroline; Gamba, Thiago de Oliveira; Flores, Isadora Luana; Oliveira, Márcia Gaiger; Rados, Pantelis Varvaki.
Afiliação
  • Vieira, Rúbia Rocha; Federal University of Rio Grande do Sul. School of Dentistry. Brazil
  • Hildebrand, Laura Campos; Federal University of Rio Grande do Sul. School of Dentistry. Brazil
  • Jesus, Luciano Henrique; Federal University of Rio Grande do Sul. School of Dentistry. Brazil
  • Klabunde, Caroline; Federal University of Rio Grande do Sul. School of Dentistry. Brazil
  • Gamba, Thiago de Oliveira; Caxia do Sul University. School of Dentristry. Brazil
  • Flores, Isadora Luana; Federal University of Rio Grande do Sul. School of Dentistry. Brazil
  • Oliveira, Márcia Gaiger; Federal University of Rio Grande do Sul. School of Dentistry. Brazil
  • Rados, Pantelis Varvaki; Federal University of Rio Grande do Sul. School of Dentistry. Brazil
Med. oral patol. oral cir. bucal (Internet) ; 25(6): e752-e761, nov. 2020. tab, graf
Artigo em Inglês | IBECS | ID: ibc-197183
Biblioteca responsável: ES1.1
Localização: BNCS
ABSTRACT

BACKGROUND:

The aim of this research was to assess the expression of aldehyde dehydrogenase 1 (ALDH1) and epithelial-mesenchymal transition (EMT) markers in head and neck squamous cell carcinoma (HNSCC), and to correlate them with the clinical and histopathological parameters of a patient cohort with follow-up over an 8-year period. MATERIAL AND

METHODS:

For this, seventeen HNSCC and non-neoplastic adjacent epithelium (AE) samples were subjected to laser microdissection and real-time PCR to evaluate the mRNA expression of ALDH1, E-cadherin (E-CAD), N-cadherin (N-CAD), and vimentin (VIM). Also, immunohistochemistry was performed for ALDH1, E-CAD, N-CAD, and VIM in the tumor center (TC), invasion front (IF), and AE of the seventeen samples. Mann-Whitney, Kruskal-Wallis and Chi-square tests were used to correlate the mRNA and immunohistochemical ex-pression with different variables, considering p < 0.05. Kaplan-Meier curves were produced for local recurrence, regional metastasis and treatment.

RESULTS:

A mRNA overexpression of ALDH1 in primary tumors was associated with regional metastasis and a high ALDH1 immunostaining was related to metastasis and a worse patient outcome. Additionally, a favorable outcome was associated with the transition phase and an unfavorable outcome was associated with EMT event. An overall 26.9 months was observed with longer survival associated with surgery and radiotherapy.

CONCLUSIONS:

However, due to the intense variability inherent to the indicator proteins in the EMT process, the complete profile markers related to this biological process should be continuous investigated
RESUMEN
No disponible
Assuntos

Texto completo: Disponível Coleções: Bases de dados nacionais / Espanha Base de dados: IBECS Assunto principal: Carcinoma de Células Escamosas / Aldeído Desidrogenase / Transição Epitelial-Mesenquimal / Neoplasias de Cabeça e Pescoço Limite: Adulto / Idoso / Feminino / Humanos / Masculino Idioma: Inglês Revista: Med. oral patol. oral cir. bucal (Internet) Ano de publicação: 2020 Tipo de documento: Artigo Instituição/País de afiliação: Caxia do Sul University/Brazil / Federal University of Rio Grande do Sul/Brazil

Texto completo: Disponível Coleções: Bases de dados nacionais / Espanha Base de dados: IBECS Assunto principal: Carcinoma de Células Escamosas / Aldeído Desidrogenase / Transição Epitelial-Mesenquimal / Neoplasias de Cabeça e Pescoço Limite: Adulto / Idoso / Feminino / Humanos / Masculino Idioma: Inglês Revista: Med. oral patol. oral cir. bucal (Internet) Ano de publicação: 2020 Tipo de documento: Artigo Instituição/País de afiliação: Caxia do Sul University/Brazil / Federal University of Rio Grande do Sul/Brazil
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