Tyrophagus putrescentiae group 4 allergen allergenicity and epitope prediction
Allergol. immunopatol
; 48(6): 619-625, nov.-dic. 2020. ilus, tab, graf
Artigo
em Inglês
| IBECS
| ID: ibc-199251
Biblioteca responsável:
ES1.1
Localização: BNCS
ABSTRACT
INTRODUCTION AND OBJECTIVES:
Allergen-specific immunotherapy (ASIT) is the only allergic disease-modifying therapy available for children and adults, and recombinant allergens are an interesting approach to improve allergy diagnosis and ASIT. Tyrophagus putrescentiae is a common storage mite that produces potent allergens. The aim of this study was to express and characterize recombinant group 4 allergen protein of T. putrescentiae (Tyr p 4), and to further investigate allergenicity and potential epitopes of Tyr p 4. MATERIALS ANDMETHODS:
The cDNA encoding Tyr p 4 was generated by RT-PCR and subcloned into pET-28a(+) plasmid. The plasmid was then transformed into E. coli cells for expression. After purification by nickel affinity chromatography and identification by SDS-PAGE, recombinant Tyr p 4 protein was used for a skin prick test and an ELISA to determine the allergic response.RESULTS:
Study participants' allergic response rate to Tyr p 4 protein was 13.3% (16/120). Eight B-cell epitopes and three T-cell epitopes of Tyr p 4 were predicted.CONCLUSIONS:
Similar to group 4 allergens of other species of mite, allergenicity of Tyr p 4 is weak. The expression, characterization and epitope prediction of recombinant Tyr p 4 protein provide a foundation for further study of this allergen in the diagnosis and ASIT of storage mite allergyRESUMEN
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Coleções:
Bases de dados nacionais
/
Espanha
Base de dados:
IBECS
Assunto principal:
Proteínas Recombinantes
/
Alérgenos
/
Dessensibilização Imunológica
/
Acaridae
/
Epitopos
Limite:
Adulto
/
Feminino
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Allergol. immunopatol
Ano de publicação:
2020
Tipo de documento:
Artigo
Instituição/País de afiliação:
Jiangsu Vocational College of Medicine/China
/
The University of Iowa, Roy J. and Lucille A/United States
/
Wuxi People's Hospital Affiliated to Nanjing Medical University/China