Cytokine-driven positive feedback loop organizes fibroblast transformation and facilitates gastric cancer progression
Clin. transl. oncol. (Print)
; 24(7): 1354-1364, julio 2022.
Artigo
em Inglês
| IBECS
| ID: ibc-203834
Biblioteca responsável:
ES1.1
Localização: ES15.1 - BNCS
ABSTRACT
BackgroundGastric cancer (GC) is a malignancy that belongs to one of the most common leading causes of cancer death. Cancer-associated fibroblasts (CAFs) promote the GC cells malignant behavior. It is still unknown how GC converts normal fibroblasts (NFs) to CAFs.MethodsGC cells were co-cultured with NFs. Bioinformatics was used to analyze the genes and signaling pathways that were changed in fibroblast. RT-PCR, western blot, and Elisa assays were used to detect the expression of cytokines in fibroblast and condition medium. Western blot and immunofluorescence demonstrated activation of relevant pathways in CAFs-like cells. Transwell, scrape, colony formation, and CCK-8 assays were performed to reveal the feedback effect of CAFs-like cells on GC cells.ResultsGC promoted the conversion of NFs to CAFs by secreting Interleukin 17A (IL-17). It included both morphological and molecular marker changes. This process was achieved by activating the nuclear factor-κB (NF-κB) pathway. On the other hand, CAFs cells could secrete C-X-C Motif Chemokine Ligand 8 (IL-8, IL-8), which promoted the malignant phenotype of GC cells. In this way, a feedback loop of mutual influence was constructed in the GC and tumor microenvironment (TME).ConclusionsOur research proved a novel model of GC-educated NFs. GC-IL-17-fibroblast-IL-8-GC axis might be a potential pathway of the interaction between GC and TME.
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Coleções:
Bases de dados nacionais
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Espanha
Base de dados:
IBECS
Assunto principal:
Neoplasias Gástricas
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Movimento Celular
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Citocinas
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Interleucina-8
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Interleucina-17
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Linhagem Celular Tumoral
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Proliferação de Células
Limite:
Humanos
Idioma:
Inglês
Revista:
Clin. transl. oncol. (Print)
Ano de publicação:
2022
Tipo de documento:
Artigo
Instituição/País de afiliação:
Liaoning Province Cancer Hospital and Institute (Cancer Hospital of China Medical University)/China