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Distinct power of bone marrow microRNA signatures and tumor suppressor genes for early detection of acute leukemia
Memari, Fatemeh; Tavakolpour, Vahid; Mohajeri, Nasrin; Poopak, Behzad; Fallah, Parviz; Alizadeh, Effat; Kouhkan, Fatemeh; Zarghami, Nosratollah.
Afiliação
  • Memari, Fatemeh; Tabriz University of Medical Sciences. Tabriz. Iran
  • Tavakolpour, Vahid; Stem Cell Technology Research Center. Tehran. Iran
  • Mohajeri, Nasrin; Tabriz University of Medical Sciences. Tabriz. Iran
  • Poopak, Behzad; Islamic Azad University of Tehran. Tehran. Iran
  • Fallah, Parviz; Checkup Clinical and Specialty Laboratory. Karaj. Iran
  • Alizadeh, Effat; Faculty of Advanced Medical Sciences. Department of Medical Biotechnology. Tabriz. Iran
  • Kouhkan, Fatemeh; Stem Cell Technology Research Center. Tehran. Iran
  • Zarghami, Nosratollah; Istanbul Aydin University. Faculty of Medicine. Istanbul. Turkey
Clin. transl. oncol. (Print) ; 24(7): 1372-1380, julio 2022. graf
Artigo em Inglês | IBECS | ID: ibc-203836
Biblioteca responsável: ES1.1
Localização: ES15.1 - BNCS
ABSTRACT
BackgroundAcute leukemia involving lymphocytic and myeloid cells is cancer with a high mortality rate. Swift and timely diagnosis might be a potential approach to improving patient prognosis and survival. The microRNA (miRNA) signatures are emerging nowadays for their promising diagnostic potential. MiRNA levels from bone marrow can be used as prognostic biomarkers.MethodsThe current study was designed to evaluate if the microRNAs and tumor suppressor genes (TSGs) profiling of hematopoietic bone marrow could help in acute leukemia early detection. Also, we assessed the DNA methyltransferase 3A (DNMT3A) expression and its possible epigenetic effects on miRNAs plus TSGs expression levels. The expression levels of ten miRNAs and four TSGs involved in acute lymphocytic leukemia (ALL) as well as acute myeloid leukemia (AML) were quantified in 43 and 40 bone marrow samples of ALL and AML patients in comparison with cancer-free subjects via real-time quantitative PCR (RT-qPCR). The receiver-operating-characteristic (ROC) analysis of miRNAs was performed in the study groups. Further, the correlation between the DNMT3A and TSGs was calculated.ResultsSignificant differences were detected in the bone marrow expression of miRNAs and TSGs (P < 0.05) between acute leukemia patients and healthy group. ROC analysis confirmed the ability of miR-30a, miR-101, miR-132, miR-129, miR-124, and miR-143 to discriminate both ALL and AML patients with an area under the ROC curve of ≥ 0.80 (P < 0.001) and high accuracy. The correlation between DNMT3A and P15/P16 TSGs revealed that DNMT3A plays a vital role in epigenetic control of TSGs expression. Our findings indicated that the downregulation of bone marrow miRNAs and TSGs was accompanied by acute leukemia development.ConclusionsThe authors conclude that this study could contribute to introducing useful biomarkers for acute leukemia diagnosis.
Assuntos


Texto completo: Disponível Coleções: Bases de dados nacionais / Espanha Base de dados: IBECS Assunto principal: Prognóstico / Medula Óssea / Leucemia Mieloide Aguda / Biomarcadores Tumorais Limite: Humanos Idioma: Inglês Revista: Clin. transl. oncol. (Print) Ano de publicação: 2022 Tipo de documento: Artigo Instituição/País de afiliação: Checkup Clinical and Specialty Laboratory/Iran / Faculty of Advanced Medical Sciences/Iran / Islamic Azad University of Tehran/Iran / Istanbul Aydin University/Turkey / Stem Cell Technology Research Center/Iran / Tabriz University of Medical Sciences/Iran

Texto completo: Disponível Coleções: Bases de dados nacionais / Espanha Base de dados: IBECS Assunto principal: Prognóstico / Medula Óssea / Leucemia Mieloide Aguda / Biomarcadores Tumorais Limite: Humanos Idioma: Inglês Revista: Clin. transl. oncol. (Print) Ano de publicação: 2022 Tipo de documento: Artigo Instituição/País de afiliação: Checkup Clinical and Specialty Laboratory/Iran / Faculty of Advanced Medical Sciences/Iran / Islamic Azad University of Tehran/Iran / Istanbul Aydin University/Turkey / Stem Cell Technology Research Center/Iran / Tabriz University of Medical Sciences/Iran
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