Zeaxanthin attenuates OVA-induced allergic asthma in mice by regulating the p38 MAPK/B-catenin signaling pathway
Allergol. immunopatol
; 50(5): 75-83, sept. 2022. ilus, graf
Artigo
em Inglês
| IBECS
| ID: ibc-208628
Biblioteca responsável:
ES1.1
Localização: ES15.1 - BNCS
ABSTRACT
Background:
Asthma is a heterogeneous and complex chronic airway disease with a high inci-dence rate, characterized by chronic airway inflammation. Although the anti-inflammatory effect of zeaxanthin has been demonstrated in various disease models, its explicit role in allergic asthma remains elusive.Methods:
An allergic asthma model was established by ovalbumin (OVA) stimulation in BALB/c nude mice. The pathological examination, collagen deposition and expression of α-smooth mus-cle actin (α-SMA) in lung tissues were determined by hematoxylin and eosin (H&E), MASSON and immunofluorescence staining, respectively. Besides, the effect of zeaxanthin on inflam-mation and oxidative stress was assessed by the enzyme-linked immunosorbent assay (ELISA) and spectrophotometry measure. Moreover, the underlying mechanism was analyzed by detect-ing the expression of phosphorylated p38 (p-p38), p38, β-catenin, p-c-Jun N-terminal kinase (p-JNK) and JNK with western blot assays.Results:
The distinct infiltration of inflammatory cells was observed in the OVA-induced asthma mice model with significantly increased concentra-tions of immunoglobulin E (IgE), interleukin-4 (IL-4), IL-5, IL-13 and eotaxin (p˂0.0 01), which were prominently reversed by zeaxanthin treatment (p˂0.001). In addition, zeaxanthin treat-ment decreased the OVA-induced collagen deposition and α-SMA expression. A similar inhibi-tory effect of zeaxanthin on the oxidative stress was also observed in the OVA-induced asthma mice model, as evidenced by the prominent decrease of malondialdehyde (MDA) concentration and the remarkable increase of superoxide dismutase (SOD), glutathione S transferase (GST) and Glutathione (GSH) concentrations (p˂0.001). Moreover, zeaxanthin introduction markedly reduced the relative expressions of p-p38/p38, β-catenin and p-JNK/JNK in the OVA-induced asthma mice model (p˂0.001) (AU)
Texto completo:
Disponível
Coleções:
Bases de dados nacionais
/
Espanha
Base de dados:
IBECS
Assunto principal:
Asma
/
Beta Catenina
/
Zeaxantinas
Limite:
Animais
Idioma:
Inglês
Revista:
Allergol. immunopatol
Ano de publicação:
2022
Tipo de documento:
Artigo
Instituição/País de afiliação:
Department of Respiration/China
/
Hangzhou Childrens Hospital/China