Transcriptomic analysis reveals process of autolysis of Kluyveromyces marxianus in vacuum negative pressure and the higher temperature
Int. microbiol
; 25(3): 515-529, Ago. 2022. ilus, graf
Artigo
em Inglês
| IBECS
| ID: ibc-216211
Biblioteca responsável:
ES1.1
Localização: ES15.1 - BNCS
ABSTRACT
Kluyveromyces marxianus is expected to be used in the production of yeast extracts due to its good fermentation ability and nutritional properties. Yeast autolysis is a key process to produce yeast extract and vacuum negative pressure stress can be used as an effective way to assist autolysis. However, the molecular mechanism of initiating Kluyveromyces marxianus autolysis induced by vacuum negative pressure and the higher temperature is still unclear. In this study, RNA-seq technology was performed mainly to analyze autolytic processes in Kluyveromyces marxianus strains. Considerable differentially expressed genes (DEGs) of downregulation were significantly enriched in 7 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways related to synthesis and transport of RNA and ribosome, which indicated that abnormal protein translations had already occurred in autolytic process. Interestingly, due to obvious change of related DEGs, endoplasmic reticulum-associated degradation (ERAD) and autophagy were activated and cell wall integrity pathway was hindered. Under the continuous influence of the external stress environment, the long-term changes of the above pathways triggered a vicious circle of gradual damage to yeast cells, which is the main cause of yeast autolysis. These results may provide important clues for the in-depth interpretation of the yeast autolytic mechanism.(AU)
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Espanha
Base de dados:
IBECS
Assunto principal:
Autólise
/
Leveduras
/
Kluyveromyces
/
Sequência de Bases
/
Fermentação
Limite:
Humanos
Idioma:
Inglês
Revista:
Int. microbiol
Ano de publicação:
2022
Tipo de documento:
Artigo
Instituição/País de afiliação:
China Three Gorges Universit/Peoples Republic of China
/
China Three Gorges University/Peoples Republic of China
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Hubei Province Angel Bioengineering Group Co/Peoples Republic of China