Effect of transduced mesenchymal stem cells with IL-10 gene on control of allergic asthma
Allergol. immunopatol
; 51(2): 45-51, 01 mar. 2023. ilus, graf
Artigo
em Inglês
| IBECS
| ID: ibc-216797
Biblioteca responsável:
ES1.1
Localização: ES15.1 - BNCS
ABSTRACT
Asthma is an important pulmonary disease associated with T helper lymphocyte (Th)2 dominant immune response, which can initiate allergic and inflammatory reactions. Interleukin (IL)-10 is the main immune suppressor cytokine, and mesenchymal stem cells (MSCs) have an immune-modulatory potential that can be transduced with the expression of the IL-10 gene to control pathophysiology of allergic asthma. Bone marrows MSCs were isolated and transduced with the expression vector that contains the expressible IL-10 gene. Then, allergic asthma mouse model was produced and treated with manipulated MSCs. Methacholine challenge test; measurement of IL-4, IL-5, IL-8, IL-13, IL-25, and IL-33; and total and ovalbumin (OVA)-specific immunoglobulin (Ig)E levels were done. Hyperplasia of the goblet cell, secretion of mucus, and peribronchiolar and perivascular eosinophilic inflammation were evaluated in lung pathological sections. IL-25, IL-33, and total IgE levels; AHR; eosinophilic inflammation; hyperplasia of the goblet cell; and secretion of mucus could be controlled in M, MV, and MV-10 groups, and the control in the MV-10 group was strong compared to M and MV groups. MSCs have immune-modulatory capacity that can control allergic asthma pathophysiology, and this effect can be strengthened and reinforced by the expression of IL-10 gene (AU)
Texto completo:
Disponível
Coleções:
Bases de dados nacionais
/
Espanha
Base de dados:
IBECS
Assunto principal:
Asma
/
Terapia Genética
/
Interleucina-10
/
Células-Tronco Mesenquimais
/
Terapia Baseada em Transplante de Células e Tecidos
/
Hipersensibilidade
Limite:
Animais
Idioma:
Inglês
Revista:
Allergol. immunopatol
Ano de publicação:
2023
Tipo de documento:
Artigo
Instituição/País de afiliação:
Henan Provincial Chest Hospital/China
/
Tehran University of Medical Sciences/Iran
/
The 7th Peoples Hospital of Zhengzhou/China
/
Zanjan University of Medical Sciences/Iran