Identification of differentially expressed genes in 4-day axolotl limb blastema by suppression subtractive hybridization
J. physiol. biochem
; 64(1): 37-50, ene.-mar. 2008. ilus, graf, tab
Artigo
em Inglês
| IBECS
| ID: ibc-61322
Biblioteca responsável:
ES1.1
Localização: BNCS
ABSTRACT
The goal of our study was the identification of up-regulated genes during axolotl(Ambystoma mexicanum) hindlimb regeneration 4 days after amputation using suppressionsubtractive hybridization (SSH). Approximately 400 clones that harboredupregulated genes in regenerating blastema tissue were selected for sequence analysis.A BLAST homology search against NCBI non-redundant database and anambystoma EST database revealed 102 clones that showed homology to knownsequences in GenBank with annotated function, 31 were known genes withoutknown function, 74 were novel and 72 belonged to mitochondrial sequences. Differentialexpression of Hmox1, Orc4L, Pls3, Fen-1, Mcm7 and Mmp3/10a was confirmedusing qRT-PCR analysis. Among all genes, only Mmp3/10a has been previouslydescribed as involved in limb regeneration. Other important identified genesbelong to the group of cell cycle regulators (Orc4L, Nasp, Skp1A and Mcm7, the latterbeing a possible proliferative marker), those involved in protein synthesis andtransport (Sec63, Srp72, Sara2) and V- ATPase pump. The novel genes we identifiedmight be important for the process of blastema formation and the onset of cell proliferationin a regenerating limb (AU)
RESUMEN
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Coleções:
Bases de dados nacionais
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Espanha
Base de dados:
IBECS
Assunto principal:
Imuno-Histoquímica
/
Expressão Gênica
/
Divisão Celular
/
Perfilação da Expressão Gênica
/
Ambystoma
/
Anfíbios
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
Idioma:
Inglês
Revista:
J. physiol. biochem
Ano de publicação:
2008
Tipo de documento:
Artigo
Instituição/País de afiliação:
University of Ljubljana/Slovenia