New and simple plate test for screening relative transfructosylation activity of fungi
Rev. iberoam. micol
; 23(3): 189-191, sept. 2006. tab, ilus
Artigo
em Inglês
| IBECS
| ID: ibc-75388
Biblioteca responsável:
ES1.1
Localização: BNCS
ABSTRACT
Several microorganisms are reported to have transfructosylation activity due to fructosyltransferase and/or fructofuranosidase activities. However, the search for other fungi with higher transfructosylation activity remains a challenge. So, a presumptive and indirect colorimetric plate assay for the evaluation of transfructosylation activity in fungi was developed which involved the simultaneous determination in the same plate of glucose and fructose released from sucrose. The method entailed the (a) glucose oxidase-peroxidase coupled reaction using phenol and 4-aminoantipyrine for determination of glucose; and (b) fructose dehydrogenase oxidation in the presence of a tetrazolium salt for determination of fructose. The presence of enzymes with transfructosylation activity was identified by the formation of pink (presence of glucose) and blue (presence of fructose) halos around the fungal colony. In conclusion, the results showed that the method is suitable for screening a large number of fungi due to its simplicity, reproducibility and rapidity and also gives a relative quantitative idea of the transfructosylation activity of different fungi species(AU)
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Coleções:
Bases de dados nacionais
/
Espanha
Base de dados:
IBECS
Assunto principal:
Microbiologia Industrial
/
Proteínas Fúngicas
/
Colorimetria
/
Fungos
/
Hexosiltransferases
/
Micologia
Tipo de estudo:
Estudo diagnóstico
/
Estudo de avaliação
/
Estudo de rastreamento
Idioma:
Inglês
Revista:
Rev. iberoam. micol
Ano de publicação:
2006
Tipo de documento:
Artigo
Instituição/País de afiliação:
Universidade do Minho/Portugal