Partial characterization of hemagglutinin activity of the marine sponge Anthosigmella varians
Braz. j. med. biol. res
; 25(1): 53-5, 1992. ilus
Artigo
em Inglês
| LILACS
| ID: lil-109000
Biblioteca responsável:
BR26.1
ABSTRACT
The marine sponge Anthosigmella varians contains proteins that agglutinate human erythrocytes irrespective of their ABO group antigens. The hemagglutination reaction depends on divalent cations andf is not inhibited by L-arabinose, D-xylose, L-rhamnose, D-galactose, D-glucose, L and D-fucose, N-acetyl-D-galactosamine, N-acetyl-D-glucosamine, methyl-alpha-Dmannopiranoside, D-cellobiose, lactose, maltose, melibiose nor raffinose (33 mM each). A partial purification of the hemagglutinins with 31-fold ioncrease in SA and 80% recovery of activity was obtained after gel filtration and ion-exchange gradient elution chromatography. Hemadsorption experiments carried our with out with the semipurified fraction using glutaraldehyde-fixed human erythrocytes suggest that protein with molecular weight of 90 and 34 kDa participate in this rection
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Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Poríferos
/
Hemaglutininas
Limite:
Animais
País/Região como assunto:
América do Sul
/
Brasil
Idioma:
Inglês
Revista:
Braz. j. med. biol. res
Assunto da revista:
Biologia
/
Medicina
Ano de publicação:
1992
Tipo de documento:
Artigo