Characterization of phosphoinositol oligosaccharides from parasitic protozoa by fast atom bombardment and collisional activation mass spectrometry
Braz. j. med. biol. res
; 27(2): 203-10, Feb. 1994. ilus
Artigo
em Inglês
| LILACS
| ID: lil-138286
Biblioteca responsável:
BR26.1
RESUMO
Fast atom bombardment mass spectrometry (FAB MS) enables the rapid, accurate and sensitive determination of the molecular masses of glycosylphosphatidylinositol(GPI)-derived oligosaccharides, from which the composition in terms of monosaccharide residues and non-carbohydrate substituents can be determined. Interpretation of fragment ions in collisional activation mass spectra further enables the determination of residue sequence, the positions of branch points, and the location of non-carbohydrate substituents. We have applied these techniques to the characterization of phosphoinositol oligosaccharides from Leptomonas samueli, Endotrypanum schaudinni and Leishmania adleri. The mass spectral data permit the postulation of candidate structures for the oligosaccharides, which provide a set of constraints that can assist the interpretation of results from other techniques such as NMR
Buscar no Google
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Oligossacarídeos
/
Fosfatidilinositóis
/
Glicolipídeos
/
Espectrometria de Massas de Bombardeamento Rápido de Átomos
/
Trypanosomatina
Idioma:
Inglês
Revista:
Braz. j. med. biol. res
Assunto da revista:
Biologia
/
Medicina
Ano de publicação:
1994
Tipo de documento:
Artigo
/
Congresso e conferência