A method to estimate mitochondrial Ca2+ uptake in intact cardiac myocytes
Braz. j. med. biol. res
; 29(12): 1699-1707, Dec. 1996. ilus
Artigo
em Inglês
| LILACS
| ID: lil-188457
Biblioteca responsável:
BR1.1
ABSTRACT
In the present paper we describe a method to estimate mitochondrial Ca2+ uptake during the declining phase of Ca2+ transients (cell relaxation) in intact isolated myocardial cells. This method is based on inhibition of sarcoplasmic reticulum (SR) Ca2+ accumulation by caffeine, blockade of Ca2+ transport via sarcolemmal Ca2+ -ATPase by treatment with carboxyeosin and inhibition of sarcolemmal Na+/Ca+ exchange by removal of extracellular Na+ and Ca2+. Ca2+ transients were evoked in rabbit ventricular myocytes by quick and sustained caffeine application (10 mM) after a 5-min period of electrical stimulation to load the SR with Ca2+. Mitochondrial Ca2+ transport was estimated using a model described by Sipido and Wier (Journal of Physiology (1991), 435 605-630), which was originally proposed to describe Ca2+ fluxes during excitation-contraction coupling in cardiac cells. Our results indicate that, in intact rabbit myocytes, the Ca2+ flux due to net mitochondrial CA2+ uptake may attain a value close to 1 muM/sec.
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Base de dados:
LILACS
Assunto principal:
Técnicas In Vitro
/
Cálcio
/
Transporte de Íons
/
Mitocôndrias Cardíacas
/
Contração Miocárdica
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Braz. j. med. biol. res
Assunto da revista:
Biologia
/
Medicina
Ano de publicação:
1996
Tipo de documento:
Artigo
/
Documento de projeto