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Regulation of transgene expression in genetic immunization
Harms, J. S; Oliveira, S. C; Splitter, G. A.
Afiliação
  • Harms, J. S; University of Wisconsin. Department of Animal Health and Biomedical Sciences.
  • Oliveira, S. C; Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia.
  • Splitter, G. A; University of Wisconsin. Department of Animal Health and Biomedical Sciences.
Braz. j. med. biol. res ; 32(2): 155-62, feb. 1999. tab, graf
Artigo em Inglês | LILACS | ID: lil-228257
Biblioteca responsável: BR1.1
RESUMO
The use of mammalian gene expression vectors has become increasingly important for genetic immunization and gene therapy as well as basic research. Essential for the success of these vectors in genetic immunization is the proper choice of a promoter linked to the antigen of interest. Many genetic immunization vectors use promoter elements from pathogenic viruses including SV40 and CMV. Lymphokines produced by the immune response to proteins expressed by these vectors could inhibit further transcription initiation by viral promoters. Our objective was to determine the effect of IFN-g on transgene expression driven by viral SV40 or CMV promoter/enhancer and the mammalian promoter/enhancer for the major histocompatibility complex class I (MHC I) gene. We transfected the luciferase gene driven by these three promoters into 14 cell lines of many tissues and several species. Luciferase assays of transfected cells untreated or treated with IFN-g indicated that although the viral promoters could drive luciferase production in all cell lines tested to higher or lower levels than the MHC I promoter, treatment with IFN-g inhibited transgene expression in most of the cell lines and amplification of the MHC I promoter-driven transgene expression in all cell lines. These data indicate that the SV40 and CMV promoter/enhancers may not be a suitable choice for gene delivery especially for genetic immunization or cancer cytokine gene therapy. The MHC I promoter/enhancer, on the other hand, may be an ideal transgene promoter for applications involving the immune system
Assuntos
Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Regulação da Expressão Gênica / Transgenes / Vacinas de DNA / Vetores Genéticos Idioma: Inglês Revista: Braz. j. med. biol. res Assunto da revista: Biologia / Medicina Ano de publicação: 1999 Tipo de documento: Artigo / Congresso e conferência / Documento de projeto
Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Regulação da Expressão Gênica / Transgenes / Vacinas de DNA / Vetores Genéticos Idioma: Inglês Revista: Braz. j. med. biol. res Assunto da revista: Biologia / Medicina Ano de publicação: 1999 Tipo de documento: Artigo / Congresso e conferência / Documento de projeto
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