Your browser doesn't support javascript.
loading
Perturbation of EGF-induced MAP kinase activation by TGF-ß1
Wenner, C. E; Yan, S.
Afiliação
  • Wenner, C. E; Roswell Park Cancer Institute. Department of Biochemistry.
  • Yan, S; Roswell Park Cancer Institute. Department of Biochemistry.
Braz. j. med. biol. res ; 32(7): 821-5, July 1999.
Artigo em Inglês | LILACS | ID: lil-234886
Biblioteca responsável: BR1.1
RESUMO
TGF-ß1 regulates both cellular growth and phenotypic plasticity important for maintaining a growth advantage and increased invasiveness in progressively malignant cells. Recent studies indicate that TGF-ß-1 stimulates the conversion of epitheliod to fibroblastoid phenotype which presumably leads to the inactivation of growth-inhibitory effects by TGF-ß1 (Portella et al. (1998) Cell Growth and Differentiation, 9 393-404). Therefore, the investigation of TGF-ß1 signaling that leads to altered growth and migration may provide novel targets for the prevention of increased cell growth and invasion. Although much attention has been paid to TGF-ß1 responses in epithelial cells, the above studies suggest that examination of signal transduction pathways in fibroblasts are important as well. Data from our laboratory are consistent with the concept that TGF-ß1 can act as a regulatory switch in density-dependent C3H 10T1/2 fibroblasts capable of either promoting or delaying G1 traverse. The regulation of this switch is proposed to occur prior to pRb phosphorylation, namely prior to activation of cyclin-dependent kinases. The current study is concerned with the evaluation of a key cyclin (cyclin D1) which activates cdk4 and p27KIP1 which in turn inhibit cdk2 in the proliferative responses of epidermal growth factor (EGF) and platelet-derived growth factor (PDGF) and their modulation by TGF-ß1. Although the molecular events that lead to elevation of cyclin D1 are not completely understood, it appears likely that activation of p42/p44MAPK kinases is involved in its transcriptional regulation. TGF-ß1 delayed EGF- or PDGF-induced cyclin D1 expression and blocked the induction of active p42/p44MAPK. The mechanism by which TGF-ß1 induces a block in p42/p44MAPK activation is being examined and the possibility that TGF-ß1 regulates phosphatase activity is being tested
Assuntos
Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Transformação Celular Neoplásica / Fator de Crescimento Transformador beta / Proteínas Quinases Dependentes de Cálcio-Calmodulina / Ciclina D1 / Fator de Crescimento Epidérmico Limite: Humanos Idioma: Inglês Revista: Braz. j. med. biol. res Assunto da revista: Biologia / Medicina Ano de publicação: 1999 Tipo de documento: Artigo / Congresso e conferência

Similares

MEDLINE

...
LILACS

LIS

Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Transformação Celular Neoplásica / Fator de Crescimento Transformador beta / Proteínas Quinases Dependentes de Cálcio-Calmodulina / Ciclina D1 / Fator de Crescimento Epidérmico Limite: Humanos Idioma: Inglês Revista: Braz. j. med. biol. res Assunto da revista: Biologia / Medicina Ano de publicação: 1999 Tipo de documento: Artigo / Congresso e conferência
...