Use of remazol blue dyed avicel for the determination of cellullolytic activity in basidiomycetes
Rev. microbiol
; 29(4): 286-8, out.-dez. 1998. graf
Artigo
em Inglês
| LILACS
| ID: lil-251738
Biblioteca responsável:
BR32.1
ABSTRACT
A modified method for direct determination of cellulolytic activity using Avicel colored with Remazol Brilliant Blue R (RBBR) in Agar test tubes in discussed. Refinaments were introduced in a simple method for qunatitation of cellulase activity, based on the release of dye from Avicel-RBBR medium by enzimatic hydrolysis. Modification in Avicel-dye preparation were enhanced and a spectrophotometer improved the precision of the collected data, since absorbance measurements could be done at the maxmum wavelenght for RBBR (595 nm).
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Basidiomycota
/
Técnicas In Vitro
/
Corantes
Idioma:
Inglês
Revista:
Rev. microbiol
Assunto da revista:
Microbiologia
Ano de publicação:
1998
Tipo de documento:
Artigo
País de afiliação:
Brasil