Polymerase chain reaction for the laboratory diagnosis of aseptic meningitis and encephalitis
Arq. neuropsiquiatr
; 58(3B): 836-42, Sept. 2000.
Artigo
em Inglês
| LILACS
| ID: lil-273108
Biblioteca responsável:
BR1.1
ABSTRACT
A protocol for testing cerebrospinal fluid specimens using a range of PCR assays for the diagnosis of central nervous system infection was developed and used to test prospectively 383 specimens. PCR assays were used for the detection of adenovirus, Borrelia burgdorferi, enteroviruses, Epstein Barr virus, cytomegalovirus, herpes simplex virus, human herpes virus type 6, JC virus, Leptospira interrogans, Listeria monocytogenes, lymphocytic choriomeningitis virus, measles virus, mumps virus, Mycobacterium sp., Mycoplasma pneumoniae, Toxoplasma gondii and varicella zoster virus. Of the 383 specimens tested in this study, 46 (12.0 percent) were found to be positive. The microorganisms detected were CMV, enterovirus, Epstein Barr virus, herpes simplex virus, human herpes virus type 6, JC virus, L. monocytogenes, Mycobacterium genus, Toxoplasma gondii and varicella zoster virus. The introduction of the PCR protocol described has improved the diagnosis of a range of central nervous system infections in our laboratory. We believe however that further evaluation of these assays in immunocompromised patients is necessary to better determine the predictive value of positive PCR results in these patient groups
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Contexto em Saúde:
Doenças Negligenciadas
Problema de saúde:
Doenças Negligenciadas
/
Zoonoses
Base de dados:
LILACS
Assunto principal:
Reação em Cadeia da Polimerase
/
Encefalite
/
Meningite Asséptica
Tipo de estudo:
Estudo diagnóstico
/
Estudo de etiologia
/
Guia de prática clínica
/
Estudo prognóstico
Limite:
Adolescente
/
Adulto
/
Criança
/
Criança, pré-escolar
/
Humanos
Idioma:
Inglês
Revista:
Arq. neuropsiquiatr
Assunto da revista:
Neurologia
/
Psiquiatria
Ano de publicação:
2000
Tipo de documento:
Artigo
País de afiliação:
Brasil
/
Reino Unido
Instituição/País de afiliação:
Public Health Laboratory/GB
/
Universidade Federal do Rio Grande do Sul/BR