Cirrose experimental induzida pela inalaçao de tetracloreto de carbono: adaptaçao da técnica e avaliaçao da peroxidaçao lipídica / Experimental cirrhosis induced by carbon tetrachloride inhalation: adaptation of the technique and evaluation of lipid peroxidation
Arq. gastroenterol
; 38(1): 40-47, Jan.-Mar. 2001.
Artigo
em Português
| LILACS
| ID: lil-290416
Biblioteca responsável:
BR1.1
RESUMO
BACKGROUND:
Long-term administration of carbon tetrachloride is an accepted experimental model to produce hepatic fibrosis. Oxidative stress has been postulated as a major molecular mechanism involved in carbon tetrachloride hepatotoxicity, where the reactive oxygen species play an important role in the pathogenesis of liver fibrosis.AIMS:
This study was conducted to evaluate the effectiveness of an experimental model of hepatic cirrhosis induced by carbon tetrachloride inhalation as well as the importance of lipid peroxidation and the characteristics of the ascitic fluid in this model.METHODS:
At first the hepatic histologic findings were assessed using the hematoxilineosin technique in different moments of carbon tetrachloride inhalation (5th, 7th, 9th, 12th weeks). Later, at the end of 15 weeks of the study the rats were divided in three groups (control; control + phenobarbital; and carbon tetrachloride + phenobarbital) for lipid peroxidation, ascitic fluid and histologic characteristics evaluation. For the lipid peroxidation analysis, thiobarbituric acid and QL techniques were used. Cytologic and bacteriologic parameters were analysed in the ascitic fluid.RESULTS:
Cirrhosis was established in 100 per cent of carbon tetrachloride rats between the 12th and 15th weeks with an elevation in the lipid peroxidation carbon tetrachloride rats' livers. Ascitic fluid infection was observed in one of seven rats who has developed ascites.CONCLUSIONS:
The carbon tetrachloride inhalation method developed in this study is effective in cirrhosis induction and ascites formation, and the carbon tetrachloride cirrhosis physiopathogenesis is probably related to the oxidative stress installation.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Líquido Ascítico
/
Tetracloreto de Carbono
/
Peroxidação de Lipídeos
/
Estresse Oxidativo
/
Fígado
/
Cirrose Hepática Experimental
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Português
Revista:
Arq. gastroenterol
Assunto da revista:
Gastroenterologia
Ano de publicação:
2001
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Fundaçäo Faculdade Federal de Ciências Médicas de Porto Alegre/BR
/
Universidade Federal do Rio Grande do Sul/BR