Carbon sources effect on pectinase production from Aspergillus japonicus 586
Braz. j. microbiol
; 31(4): 286-290, oct.-dec. 2000. tab, graf
Artigo
em Inglês
| LILACS
| ID: lil-299826
Biblioteca responsável:
BR32.1
ABSTRACT
The effect of different carbon sources on the pectinesterases, endo- and exo-polygalacturonase activities from Aspergillus japonicus 586 was evaluated in liquid media (Manachini solutions) supplemented with different substrate concentrations. The culture medium was inoculated with 5.106 spores/ml and mantained under agitation (140 rpm), at 30ºC, during 122 h. The enzyme evaluation was carried out 24 h after filtration. The crude extract from A. japonicus 586 indicated that the best enzymatic activities were afforded in the presence of 0.5 per cente pectin (pectinesterease), 0.2 per cente pectin and 0.2 per cente glycerol (endopolygalacturonase), and 0.5per cente pectin associated to 0.5 per cente glucose (exopolygalacturonase). Carbon sources concentration, isolated or associated, significantly affects the pectinesterase, and endo- and exopolygalacturonase activities. Pectin, glucose and saccharose, when added to the culture medium in high concentrations, exhibited a repression effect on all the analyzed enzymes.(au)
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Poligalacturonase
/
Aspergillus
/
Técnicas In Vitro
/
Carbono-Carbono Ligases
/
Enzimas
Idioma:
Inglês
Revista:
Braz. j. microbiol
Assunto da revista:
Microbiologia
Ano de publicação:
2000
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Universidade Estudual de Campinas/BR
/
Universidade de Amazonas/BR
/
Universidade de Pernambuco/BR