New insights to the functional role of the T cell-Antigen presenting cell immunological synapse
Biol. Res
; 35(2): 133-137, 2002.
Artigo
em Inglês
| LILACS
| ID: lil-323335
Biblioteca responsável:
BR1.1
ABSTRACT
Three innovative and complementary morphological approaches were employed to study the T cell/antigen presenting cell (APC) interaction (i) high resolution three-dimensional confocal microscopy of the T cell-APC contact site; (ii) time lapse video recording in living T cells of [Ca2+]I and changes in distribution of various GFP fusion proteins with TCR/CD3-zeta complex associated- and other signaling components; (iii) measurement of lateral TCR mobility and that of recruited signaling components using techniques based on fluorescence recovery after photo-bleaching. These approaches were combined with biochemical and functional experiments to investigate two principal issues (A) Recruitment and the three-dimensional arrangement of receptors and signaling components at the contact site between human CD4+ T lymphocytes and APCs, (B) Structure of the immunological synapse formed at the contact site between cytotoxic T lymphocytes (CTLs) and target cells. We discuss evidence indicating that TCR engagement and triggering can occur in the absence of large-scale molecular segregation into the T cell-APC contact site. Taken together our results indicate that although not required for TCR engagement and triggering, formation of the IS is important to reinforce TCR-mediated signal transduction and achieve full T cell activation
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Receptores de Antígenos de Linfócitos T
/
Linfócitos T CD4-Positivos
/
Linfócitos T CD8-Positivos
/
Células Apresentadoras de Antígenos
Limite:
Animais
/
Humanos
Idioma:
Inglês
Revista:
Biol. Res
Assunto da revista:
Biologia
Ano de publicação:
2002
Tipo de documento:
Artigo
País de afiliação:
França
Instituição/País de afiliação:
Institut Claude de Prével/FR