Kinetics of T cell-activation molecules in response to Mycobacterium tuberculosis antigens
Mem. Inst. Oswaldo Cruz
; 97(8): 1097-1099, Dec. 15, 2002. tab, graf
Artigo
em Inglês
| LILACS
| ID: lil-326339
Biblioteca responsável:
BR1.1
RESUMO
The phenotypic features acquired subsequent to antigen-specific stimulation in vitro were evaluated by means of the kinetic expressions of CD69 and CD25 activation molecules on T lymphocytes and assayed by flow cytometry in response to PPD, Ag85B, and ferritin in PPD-positive healthy control individuals. In response to PHA, CD69 staining on both CD4+ and CD8+ T cells became initially marked after 4 h, peaked at 24 h, and quickly decreased after 120 h. For CD25, a latter expression was detected around 8 h, having increased after 96 h. As expected, the response rate to the mycobacterial antigens was much lower than that to the mitogen. Positive staining was high after 96 h for CD25 and after 24 h for CD69. CD69 expression was significantly enhanced (p < 0.05) on CD8+ as compared to CD4+ T cells. High levels were also found between 96-120 h. Regarding Ag85B, CD25+ cells were mostly CD4+ instead of CD8+ T cells. Moreover, in response to ferritin, a lower CD25 expression was noted. The present data will allow further characterization of the immune response to new mycobacterial-specific antigens and their evaluation for possible inclusion in developing new diagnostic techniques for tuberculosis as well in a new vaccine to prevent the disease
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Contexto em Saúde:
ODS3 - Saúde e Bem-Estar
/
Doenças Negligenciadas
Problema de saúde:
Meta 3.3: Acabar com as doenças tropicais negligenciadas e combater as doenças transmissíveis
/
Doenças Negligenciadas
/
Tuberculose
Base de dados:
LILACS
Assunto principal:
Ativação Linfocitária
/
Linfócitos T CD4-Positivos
/
Receptores de Interleucina-2
/
Antígenos de Diferenciação de Linfócitos T
/
Antígenos CD
/
Linfócitos T CD8-Positivos
/
Mycobacterium tuberculosis
/
Antígenos de Bactérias
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
2002
Tipo de documento:
Artigo
País de afiliação:
Brasil
/
Holanda
Instituição/País de afiliação:
Fiocruz/BR
/
Hospital Municipal Raphael de Paula e Souza/BR
/
Leiden University Medical Center/NL
/
Royal Tropical Institute/NL