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No mutations found in exons of TP53, H-RAS and K-RAS genes in liver of male Wistar rats submitted to a medium-term chemical carcinogenesis assay
Castelli, Erik da Cruz; Otake, Andréia Hanada; Oliveira, Deilson Elgui de; Rocha, Noeme Souza; Salvadori, Daisy Maria Fávero; Camargo, João Lauro Viana de.
Afiliação
  • Castelli, Erik da Cruz; s.af
  • Otake, Andréia Hanada; s.af
  • Oliveira, Deilson Elgui de; s.af
  • Rocha, Noeme Souza; Universidade Estadual Paulista. Departamento de Clínica Médica e Patologia. BR
  • Salvadori, Daisy Maria Fávero; Universidade Estadual Paulista. Departamento de Patologia. BR
  • Camargo, João Lauro Viana de; Universidade Estadual Paulista. Departamento de Patologia. BR
J. bras. patol. med. lab ; 38(3): 175-182, jul.-set. 2002. ilus, tab
Artigo em Inglês | LILACS | ID: lil-330640
Biblioteca responsável: BR14.1
RESUMO
The standart protocol to evaluate the carcionogenic potencial of chemicals is the long-term bioassay in rodents, not performed in developing countries due to its high cost and complex operational procedures. Our laboratory has established an alternative an alternative medium-term bioassay in Wistar rats, also DMBDD assay, based on the paradigm iniation/promotion of chemical carcinogenesis. This method was accepted by the Brazilian Environment Agency (IBAMA) as an official source of evidence of carcinogenity. The aim of this study was to evaluate alterations in exons 5 to 8 of the tumor suppressor gene TP53 and exons 1 and 2 of oncogenes K-RAS and H-RAS in preneoplastic hepatic lesions observed in DMBDD assay. The characterization of these alterations may contribute to the recognition of patterns of damage in critical genes, as well as to suggest mechanisms of action of the compounds tested in the protocol. Sixty male wistar rats were separeted into3 groups? the first was treated with no chemicals; the second received five initiaing agents and the third received initiation followed by phenobarbital. Liver DNA samples (obtained from formalin-fixed and parafin-fixed and paraffin-embedded tissues after histological analysis) were evaluated by the non-isotopic PCR-SSCP technique. No changes in any analyzed exons were detected by the PCR-SSCP banding pattern in all experimental groups. This suggests that liver in exons 5 to 8 of TP53 and exons 1 and 2 of H-RAS are not among the early molecular alterations occuring in the hepatic carcinogenesis process by the DMBDD protocol in male Wistar rats
Assuntos
Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Carcinógenos / Mutagênese / Ratos Wistar / Polimorfismo Conformacional de Fita Simples / Neoplasias Hepáticas Experimentais / Mutação Limite: Animais Idioma: Inglês Revista: J. bras. patol. med. lab Assunto da revista: Patologia Ano de publicação: 2002 Tipo de documento: Artigo País de afiliação: Brasil Instituição/País de afiliação: Universidade Estadual Paulista/BR

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Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Carcinógenos / Mutagênese / Ratos Wistar / Polimorfismo Conformacional de Fita Simples / Neoplasias Hepáticas Experimentais / Mutação Limite: Animais Idioma: Inglês Revista: J. bras. patol. med. lab Assunto da revista: Patologia Ano de publicação: 2002 Tipo de documento: Artigo País de afiliação: Brasil Instituição/País de afiliação: Universidade Estadual Paulista/BR
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