Characterization of enteroaggregative Escherichia coli strains isolated from children with acute diarrhea, in Mendoza, Argentina
Rev. argent. microbiol
; 34(3): 167-170, jul.-sept. 2002.
Artigo
em Inglês
| LILACS
| ID: lil-331787
Biblioteca responsável:
BR1.1
RESUMO
Enteroaggregative Escherichia coli (EAEC) is an increasingly recognized cause of diarrhea in children in developing and developed countries. EAEC is recognized by a characteristic aggregative pattern of adherence to human epithelial (HEp-2) cells cultured in vitro. This is the gold standard assay. The aggregative phenotype is associated with the presence of a 65 MDa plasmid (pAA) that also encodes several other putative virulence factors, such as the aggregative adherence fimbria I (AAF/I) and the enteroaggregative heat-stable enterotoxin (EAST1). The objective of this work was to evaluate the application of PCR (polymerase chain reaction) to identify EAEC strains in cases of acute diarrhea. A total of 87 E. coli strains, isolated from patients under 2 years of age with acute diarrhea in Mendoza, Argentina, were characterized by the reference method (HEp-2 assay), and by AAF/I- and EAST1-PCR. PCR sensitivity and specificity in comparison with the cell culture assay showed 94.4 sensitivity and 78.26 specificity. EAST1- and AAF/I-PCR could be recommended as a screening test, applicable to epidemiologic studies.
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Coleções:
Bases de dados internacionais
Contexto em Saúde:
ODS3 - Saúde e Bem-Estar
/
Doenças Negligenciadas
/
ODS3 - Meta 3.3 Acabar com as doenças tropicais negligenciadas e combater as doenças transmissíveis
Problema de saúde:
Meta 3.3: Acabar com as doenças tropicais negligenciadas e combater as doenças transmissíveis
/
Diarreia
/
Doenças Negligenciadas
/
Zoonoses
/
Infecções Diarreicas
Base de dados:
LILACS
Assunto principal:
Aderência Bacteriana
/
Diarreia Infantil
/
Escherichia coli
/
Infecções por Escherichia coli
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
/
Estudo de rastreamento
Limite:
Humanos
/
Lactente
País/Região como assunto:
América do Sul
/
Argentina
Idioma:
Inglês
Revista:
Rev. argent. microbiol
Assunto da revista:
Microbiologia
Ano de publicação:
2002
Tipo de documento:
Artigo
País de afiliação:
Argentina
Instituição/País de afiliação:
Hospital Materno Infantil Dr. Humberto Notti/AR
/
Universidad Nacional de Cuyo/AR
/
Universidad de San Luis/AR