Actividad semejante a catepsina L en la hemolinfa de hembras repletas de Boophilus microplus / Cathepsin L-like activity in hemolymph of females crammed with Boophilus microplus
Rev. cuba. med. trop
; 51(3): 201-203, Sept.-Dec. 1999.
Artigo
em Espanhol
| LILACS
| ID: lil-333507
Biblioteca responsável:
BR1.1
ABSTRACT
The cysteine-proteinase activity present in the hemolymph of females crammed with Boophilus microplus at the second oviposition was proved. The enzyme assay was performed using the synthetic N-carbobenzoxi-phenilalanyl-arginil-4-metoxibetan++ +-naphthylamide substrate, and hydrolysis in both raw ovarim extract and hemolymph were observed. The activity was 100 inhibited by trans-epoxisuscinyl-L-leucidamide (4-guadinine) butane, an specific inhibitor for cysteine-proteinase. Polyacrylamide and gelatin-gel electrophoresis showed the existence of only one active band in both samples tested with the same electrophoretic sliding (approximately 38 kDa).
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Parasitos
/
Hemolinfa
/
Catepsinas
Limite:
Animais
Idioma:
Espanhol
Revista:
Rev. cuba. med. trop
Assunto da revista:
Medicina Tropical
Ano de publicação:
1999
Tipo de documento:
Artigo
País de afiliação:
Cuba
Instituição/País de afiliação:
Instituto de Medicina Tropical Pedro Kouri/CU