Estudio de algunas propiedades biológicas de 3 cepas de dengue 2 con diferencias en sus secuencias nucleotídicas / Study of various biological properties of 3 strains of dengue 2 with differences in their nucleotide sequences
Rev. cuba. med. trop
; 51(3): 177-180, Sept.-Dec. 1999.
Artigo
em Espanhol
| LILACS
| ID: lil-333512
Biblioteca responsável:
BR1.1
RESUMO
Some biological properties of Dengue-2 strains such as A-15 (isolated in Cuba in 1981); Jamaica (isolated in Jamaica in 1981) and New Guinea "C" (NG"C") standard strain differing in their nucleotide sequences were studied. The results showed that the cytopathic effect in C6/36 HT cell line occurred earlier in A-15 strain and that fluorescence was first detected in Jamaica and A-15 strains. This seems to indicate that rapid detection of strains does not have any relation to neither their history of passage nor the original isolation system. A-15 and NG"C" strains exhibited an heterogeneous pattern formed by big and small plaques but average size of plaques in NG"C" was lower whereas Jamaica showed only small plaques. The most neurovirulent strain in mice was NG"G" followed by A-15 whereas Jamaica was not neurovirulent at all. These results indicate that A-15 has a different biological behaviour which is probably due to intrinsic differences. It should be taken into account that 7 amino acid changes were found in the envelope protein which may have affected the expression of some biological properties.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Contexto em Saúde:
Doenças Negligenciadas
Problema de saúde:
Dengue
Base de dados:
LILACS
Assunto principal:
DNA Viral
/
Vírus da Dengue
Limite:
Animais
Idioma:
Espanhol
Revista:
Rev. cuba. med. trop
Assunto da revista:
Medicina Tropical
Ano de publicação:
1999
Tipo de documento:
Artigo
País de afiliação:
Cuba
/
Venezuela
Instituição/País de afiliação:
Instituto de Medicina Tropical Pedro Kouri/CU
/
Laboratorio Regional de diagnóstico e investigaciones del dengue y otras enfermedades virales/VE