5-Aminolevulinic acid and the hepatic oxidative stress in the early phase of experimental hexachlorobenzene intoxication
RBCF, Rev. bras. ciênc. farm. (Impr.)
; 40(2): 203-208, abr.-jun. 2004. tab, graf
Artigo
em Inglês
| LILACS
| ID: lil-391026
Biblioteca responsável:
BR40.1
RESUMO
This work evaluated the levels of 5-aminolevulinic acid (ALA) in the liver of rats exposed to different doses of HCB (25,50, and 100mg/kg b.w. for 4 weeks) and correlated them with lipid peroxidation parameters. Levels of ALA were determined by highpressure liquid chromatography after derivatization with acetylacetone and formaldehyde, followed by fluorescence detection. The methodology was carefully validated, nonetheless hepatic levels of ALA in all animals treated or not were below the detection limit of the method (2.27mg of ALA/g liver). On the other hand, lipid peroxidation, evaluated as thiobarbituric acid reactants production and chemiluminescence was found significantly increased in the livers of all treated rats, in comparison with control values (p<0.05). These results suggest that the hepatic oxidative stress observed in animals following HCB treatment may not necessarily be associated with increased levels of ALA in the liver. Another possibility is that increased levels of ALA in the liver, even below the detection limit of the method are sufficient to induce hepatic oxidative stress
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Peroxidação de Lipídeos
/
Estresse Oxidativo
/
Ácido Aminolevulínico
/
Hexaclorobenzeno
Idioma:
Inglês
Revista:
RBCF, Rev. bras. ciênc. farm. (Impr.)
Assunto da revista:
Bioquímica
/
Farmácia
/
Farmacologia
Ano de publicação:
2004
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Universidade de São Paulo/BR