Efficacy of an enzyme-linked immunosorbent assay as a diagnostic tool for schistosomiasis mansoni in individuals with low worm burden
Mem. Inst. Oswaldo Cruz
; 100(4): 421-425, July 2005. tab
Artigo
em Inglês
| LILACS
| ID: lil-405998
Biblioteca responsável:
BR1.1
RESUMO
IgM-ELISA is an immunoenzymatic method useful for detection of IgM antibodies against a fraction of Schistosoma mansoni adult worm antigen (AWA) that is soluble in trichloroacetic acid (AWA-TCA). This method was applied to three groups of individuals with different clinical and epidemiological characteristics, and the results compared with those obtained by other diagnostic methods:
immunofluorescence test for detection of IgM antibodies (IgM-IFT) or IgG antibodies (IgG-IFT), ELISA for detection of IgG antibodies (IgG-ELISA), and two parasitological methods, Kato-Katz and miracidium hatching. The IgM-ELISA presented a sensitivity of 98 percent, when the parasitologic fecal examination was defined as reference diagnostic method, and a specificity of 98 and 97.3 percent, respectively for the group of clinically healthy individuals and other helminth carriers. A comparative analysis between the results of IgM-ELISA and those obtained by other serologic tests showed a good degree of agreement, with Kappa indices ranging from 0.95 to 0.98. The diagnostic efficacy of 97.8 percent, as determined with schistosomiasis patients with low parasitic burden, suggests the excellent performance of the IgM-ELISA and its usefulness for the diagnosis of schistosomiasis when applied in low endemic areas.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Contexto em Saúde:
Doenças Negligenciadas
Problema de saúde:
Helmintíase
/
Doenças Negligenciadas
/
Esquistossomose
/
Zoonoses
Base de dados:
LILACS
Assunto principal:
Schistosoma mansoni
/
Esquistossomose mansoni
/
Imunoglobulina G
/
Imunoglobulina M
/
Ensaio de Imunoadsorção Enzimática
/
Anticorpos Anti-Helmínticos
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
/
Humanos
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
2005
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Brasil
Instituição/País de afiliação:
Instituto de Medicina Tropical de São Paulo/BR
/
Universidade de São Paulo/BR
/
Universidade de Taubaté/BR