Designing and validation of genus-specific primers for human gut flora study
Electron. j. biotechnol
; 9(5)Oct. 2006. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-451674
Biblioteca responsável:
CL1.1
ABSTRACT
The aim of this study, was to design and validate 16S rRNA targeted oligonucleotide genus specific primers for amplifying the predominant members of gut flora using polymerase chain reaction. Primers were validated against human faecal samples. Gut flora of a normal individual was compared with that of two diseased individuals. Our observations showed that the genera Lactobacillus, Bacteroides, Peptococcus, Bifidobacterium, and E. coli were invariably present in all studied subjects however, the absence of butyrate producing bacteria Ruminococcus and Peptostreptococcus were significant. Presence of the members of the genus, Campylobacter in both the diseased samples were also unusual.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Idioma:
Inglês
Revista:
Electron. j. biotechnol
Assunto da revista:
Biotecnologia
Ano de publicação:
2006
Tipo de documento:
Artigo
País de afiliação:
Índia
Instituição/País de afiliação:
Jamia Millia Islamia/IN
/
Jawaharlal Nehru University/IN