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Designing and validation of genus-specific primers for human gut flora study
Rekha, Rani; Alam Rizvi, Moshahid; Jaishree, Paul.
Afiliação
  • Rekha, Rani; Jawaharlal Nehru University. School of Life Sciences. New Delhi. IN
  • Alam Rizvi, Moshahid; Jamia Millia Islamia. Department of Biosciences. New Delhi. IN
  • Jaishree, Paul; Jawaharlal Nehru University. School of Life Sciences. New Delhi. IN
Electron. j. biotechnol ; 9(5)Oct. 2006. ilus, tab
Artigo em Inglês | LILACS-Express | LILACS | ID: lil-451674
Biblioteca responsável: CL1.1
ABSTRACT
The aim of this study, was to design and validate 16S rRNA targeted oligonucleotide genus specific primers for amplifying the predominant members of gut flora using polymerase chain reaction. Primers were validated against human faecal samples. Gut flora of a normal individual was compared with that of two diseased individuals. Our observations showed that the genera Lactobacillus, Bacteroides, Peptococcus, Bifidobacterium, and E. coli were invariably present in all studied subjects however, the absence of butyrate producing bacteria Ruminococcus and Peptostreptococcus were significant. Presence of the members of the genus, Campylobacter in both the diseased samples were also unusual.
Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Idioma: Inglês Revista: Electron. j. biotechnol Assunto da revista: Biotecnologia Ano de publicação: 2006 Tipo de documento: Artigo País de afiliação: Índia Instituição/País de afiliação: Jamia Millia Islamia/IN / Jawaharlal Nehru University/IN

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Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Idioma: Inglês Revista: Electron. j. biotechnol Assunto da revista: Biotecnologia Ano de publicação: 2006 Tipo de documento: Artigo País de afiliação: Índia Instituição/País de afiliação: Jamia Millia Islamia/IN / Jawaharlal Nehru University/IN
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