Plasmodium vivax thrombospondin related adhesion protein: immunogenicity and protective efficacy in rodents and Aotus monkeys
Mem. Inst. Oswaldo Cruz
; 102(3): 411-416, June 2007. tab, ilus
Artigo
em Inglês
| LILACS
| ID: lil-452521
Biblioteca responsável:
BR1.1
ABSTRACT
The thrombospondin related adhesion protein (TRAP) is a malaria pre-erythrocytic antigen currently pursued as malaria vaccine candidate to Plasmodium falciparum. In this study, a long synthetic peptide (LSP) representing a P. vivax TRAP fragment involved in hepatocyte invasion was formulated in both Freund and Montanide ISA 720 adjutants and administered by IM and subcutaneous routes to BALB/c mice and Aotus monkeys. We measured specific humoral immune responses in both animal species and performed a sporozoite challenge in Aotus monkeys to assess the protective efficacy of the vaccine. After immunization both mice and Aotus seroconverted as shown by ELISA, and the specific anti-peptide antibodies cross reacted with the parasite in IFAT assays. Only two out of six immunized animals became infected after P. vivax sporozoite challenge as compared with four out of six animals from the control group. These results suggest that this TRAP fragment has protective potential against P. vivax malaria and deserves further studies as vaccine candidate.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Contexto em Saúde:
Doenças Negligenciadas
Problema de saúde:
Malária
Base de dados:
LILACS
Assunto principal:
Plasmodium vivax
/
Vacinas Sintéticas
/
Proteínas de Protozoários
/
Malária Vivax
/
Vacinas Antimaláricas
Limite:
Animais
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
2007
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Colômbia
/
Suíça
Instituição/País de afiliação:
Universidad San Martin/CO
/
Universidad del Valle/CO
/
University of Lausanne/CH