Evaluation of different DNA-based fingerprinting methods for typing Photobacterium damselae ssp. piscicida
Biol. Res
; 40(1): 85-92, 2007. ilus, tab
Article
em En
| LILACS
| ID: lil-456611
Biblioteca responsável:
BR1.1
ABSTRACT
This study evaluates the effectiveness of three different molecular techniques, repetitive extragenic palindromic PCR (REP-PCR), enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR) and the random amplified polymorphic DNA (RAPD-PCR) for rapid typing of Photobacterium damselae ssp. piscicida strains isolated from different species of marine fish and geographic areas. The results obtained by the three methods showed that RAPD and ERIC-PCR were more discriminative for suitable rapid typing of Ph. damselae ssp. piscicida than REP-PCR. The analysis of DNA banding patterns generated by both molecular methods (RAPD and ERIC-PCR) clearly separated the strains into two main groups that strongly correlated with their geographic origin. Moreover, the REP-PCR analysis was less reproducible than the RAPD and ERIC-PCR methods and does not allow the establishment of genetic groups. RAPD and ERIC-PCR constitute valuable tools for molecular typing of Ph. damselae ssp. piscicida strains, which can be used in epidemiological studies of photobacteriosis infections.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Photobacterium
/
Variação Genética
/
Impressões Digitais de DNA
/
Técnicas de Tipagem Bacteriana
Limite:
Animals
Idioma:
En
Revista:
Biol. Res
Assunto da revista:
BIOLOGIA
Ano de publicação:
2007
Tipo de documento:
Article
/
Project document
País de afiliação:
Itália
/
Espanha
País de publicação:
Chile