Genetic variability of Aedes aegypti in the Americas using a mitochondrial gene: evidence of multiple introductions
Mem. Inst. Oswaldo Cruz
; 102(5): 573-580, Aug. 2007. tab, graf, ilus
Artigo
em Inglês
| LILACS, Sec. Est. Saúde SP
| ID: lil-458624
Biblioteca responsável:
BR1.1
ABSTRACT
To analyze the genetic relatedness and phylogeographic structure of Aedes aegypti, we collected samples from 36 localities throughout the Americas (Brazil, Peru, Venezuela, Guatemala, US), three from Africa (Guinea, Senegal, Uganda), and three from Asia (Singapore, Cambodia, Tahiti). Amplification and sequencing of a fragment of the mitochondrial NADH dehydrogenase subunit 4 gene identified 20 distinct haplotypes, of which 14 are exclusive to the Americas, four to African/Asian countries, one is common to the Americas and Africa, and one to the Americas and Asia. Nested clade analysis (NCA), pairwise distribution, statistical parsimony, and maximum parsimony analyses were used to infer evolutionary and historic processes, and to estimate phylogenetic relationships among haplotypes. Two clusters were found in all the analyses. Haplotypes clustered in the two clades were separated by eight mutational steps. Phylogeographic structure detected by the NCA was consistent with distant colonization within one clade and fragmentation followed by range expansion via long distance dispersal in the other. Three percent of nucleotide divergence between these two clades is suggestive of a gene pool division that may support the hypothesis of occurrence of two subspecies of Ae. aegypti in the Americas.
Texto completo:
Disponível
Coleções:
Bases de dados nacionais
/
Brasil
Contexto em Saúde:
Doenças Negligenciadas
Problema de saúde:
Dengue
Base de dados:
LILACS
/
Sec. Est. Saúde SP
Assunto principal:
Variação Genética
/
DNA Mitocondrial
/
Aedes
/
Genética Populacional
/
Insetos Vetores
/
NADH Desidrogenase
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
País/Região como assunto:
África
/
Ásia
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Ano de publicação:
2007
Tipo de documento:
Artigo
/
Documento de projeto
Instituição/País de afiliação:
Fiocruz/BR
/
Universidade de São Paulo/BR