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Apoptosis related genes expressed in cultured Fallopian tube epithelial cells infected in vitro with Neisseria gonorrhoeae
Reyes, Paz A; Vargas, Macarena F; García, Katherine P; Rubilar, Paulina S; Navarrete, Patricia A; Fuentes, Pamela M; Ríos, Miguel A; Orihuela, Pedro A; Vargas, Renato H; Rubio, Víctor H; Heckels, John; Christodoulides, Myron; Cárdenas, Hugo; Velasquez, Luis A.
Afiliação
  • Reyes, Paz A; Universidad de Santiago de Chile. Facultad de Química y Biología. Laboratorio de Inmunología de la Reproducción. Santiago. CL
  • Vargas, Macarena F; Universidad de Santiago de Chile. Facultad de Química y Biología. Laboratorio de Inmunología de la Reproducción. Santiago. CL
  • García, Katherine P; Universidad de Santiago de Chile. Facultad de Química y Biología. Laboratorio de Inmunología de la Reproducción. Santiago. CL
  • Rubilar, Paulina S; Universidad de Santiago de Chile. Facultad de Química y Biología. Laboratorio de Inmunología de la Reproducción. Santiago. CL
  • Navarrete, Patricia A; Universidad de Santiago de Chile. Facultad de Química y Biología. Laboratorio de Inmunología de la Reproducción. Santiago. CL
  • Fuentes, Pamela M; Universidad de Santiago de Chile. Facultad de Química y Biología. Laboratorio de Inmunología de la Reproducción. Santiago. CL
  • Ríos, Miguel A; Universidad de Santiago de Chile. Facultad de Química y Biología. Laboratorio de Inmunología de la Reproducción. Santiago. CL
  • Orihuela, Pedro A; Universidad de Santiago de Chile. Facultad de Química y Biología. Laboratorio de Inmunología de la Reproducción. Santiago. CL
  • Vargas, Renato H; Hospital San José. Santiago. CL
  • Rubio, Víctor H; Hospital San José. Santiago. CL
  • Heckels, John; University of Southampton Medical School. Division of Infection, Inflammation and Repair. Molecular Microbiology and Infection. Southampton. GB
  • Christodoulides, Myron; University of Southampton Medical School. Division of Infection, Inflammation and Repair. Molecular Microbiology and Infection. Southampton. GB
  • Cárdenas, Hugo; Universidad de Santiago de Chile. Facultad de Química y Biología. Laboratorio de Inmunología de la Reproducción. Santiago. CL
  • Velasquez, Luis A; Universidad de Santiago de Chile. Facultad de Química y Biología. Laboratorio de Inmunología de la Reproducción. Santiago. CL
Biol. Res ; 40(3): 319-327, 2007. ilus, tab
Artigo em Inglês | LILACS | ID: lil-481309
Biblioteca responsável: BR1.1
ABSTRACT

Background:

Infection of the Fallopian tubes (FT) by Neisseria gonorrhoeae (Ngo) can lead to acute salpingitis, an inflammatory condition resulting in damage primarily to the ciliated cells, with loss of ciliary activity and sloughing of the cells from the epithelium. Recently, we have shown that Ngo infection induced apoptosis in FT epithelium cells by a TNF-alpha dependent mechanism that could contribute to the cell and tissue damage observed in gonococcal salpingitis.

Aim:

To investigate the apoptosis-related genes expressed during apoptosis induction in cultured FT epithelial cells infected in vitro by Ngo. Materials and

Methods:

In the current study, we used cDNA macroarrays and real time PCR to identify and determine the expression levels of apoptosis related genes during the in vitro gonococci infection of FT epithelial cells.

Results:

Significant apoptosis was induced following infection with Ngo. Macroarray analysis identified the expression of multiple genes of the TNF receptor family (TNFRSF1B, -4, -6, -10A, -10B and -10D) and the Bcl-2 family (BAK1, BAX, BLK, HRK and MCL-1) without differences between controls and infected cells. This lack of difference was confirmed by RT-PCR of BAX, Bcl-2, TNFRS1A (TNFR-I) and TNFRSF1B (TNFR-II).

Conclusion:

Several genes related to apoptosis are expressed in primary cultures of epithelial cells of the human Fallopian tube. Infection with Ngo induces apoptosis without changes in the pattern of gene expression of several apoptosis-related genes. Results strongly suggest that Ngo regulates apoptosis in the FT by post-transcriptional mechanisms that need to be further addressed.
Assuntos

Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Salpingite / Apoptose / Células Epiteliais / Tubas Uterinas / Neisseria gonorrhoeae Tipo de estudo: Estudo prognóstico Limite: Feminino / Humanos Idioma: Inglês Revista: Biol. Res Assunto da revista: Biologia Ano de publicação: 2007 Tipo de documento: Artigo / Documento de projeto País de afiliação: Chile / Reino Unido Instituição/País de afiliação: Hospital San José/CL / Universidad de Santiago de Chile/CL / University of Southampton Medical School/GB
Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Salpingite / Apoptose / Células Epiteliais / Tubas Uterinas / Neisseria gonorrhoeae Tipo de estudo: Estudo prognóstico Limite: Feminino / Humanos Idioma: Inglês Revista: Biol. Res Assunto da revista: Biologia Ano de publicação: 2007 Tipo de documento: Artigo / Documento de projeto País de afiliação: Chile / Reino Unido Instituição/País de afiliação: Hospital San José/CL / Universidad de Santiago de Chile/CL / University of Southampton Medical School/GB
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