Pharmacological characterization of venoms obtained from Mexican toxoglossate gastropods on isolated guinea pig ileum
J. venom. anim. toxins incl. trop. dis
; 14(3): 497-513, 2008. graf, tab
Artigo
em Inglês
| LILACS
| ID: lil-492212
Biblioteca responsável:
BR33.1
ABSTRACT
The protein-containing extracts prepared from the venom ducts of Conus austini, Conus spurius and Polystira albida caused a concentration-dependent inhibition of spontaneous contractions in guinea pig ileum. The most potent extract was obtained from P. albida venom ducts (IC50 equal 0.11 more or less 0.02 microg protein/mL). The three extracts produced a moderate inhibition of contractions elicited by acetylcholine (ACh 1 microM), suggesting the presence of anticholinergic compounds. The contractile response elicited by nicotine (10 microM) was significantly reduced by the extracts prepared from the ducts of C. austini and P. albida, which indicates that the venom produced by these species contains toxins that target neuronal nicotinic receptors. All three extracts significantly inhibited contractions evoked by histamine (0.5 miM), particularly those from C. spurius and P. albida. These findings reveal the presence of antihistaminergic compounds not previously described in any conoidean venom. Finally, we found that only the extract prepared from C. spurius ducts decreased KCl (60 mM)-induced contractions, indicating that the venom of this snail contains compounds that block voltage-dependent Ca2 more or Na more channels.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Cobaias
/
Íleo
/
Venenos de Moluscos
Limite:
Animais
País/Região como assunto:
México
Idioma:
Inglês
Revista:
J. venom. anim. toxins incl. trop. dis
Assunto da revista:
Toxicologia
Ano de publicação:
2008
Tipo de documento:
Artigo
País de afiliação:
México
Instituição/País de afiliação:
Autonomus University of Querétaro/MX
/
National Autonomous University of Mexico/MX