HSV-1-derived amplicon vectors: recent technological improvements and remaining difficulties: a review
Mem. Inst. Oswaldo Cruz
; 104(3): 399-410, May 2009. ilus
Artigo
em Inglês
| LILACS
| ID: lil-517020
Biblioteca responsável:
BR1.1
ABSTRACT
Amplicons are defective and non-integrative vectors derived from herpes simplex virus type 1. As the vector genome carries no virus genes, amplicons are both non-toxic for the infected cells and non-pathogenic for the inoculated organisms. In addition, the large transgenic capacity of amplicons, which allow delivery of up to 150 Kbp of foreign DNA, makes these vectors one of the most powerful, interesting and versatile gene delivery platforms. We present here recent technological developments that have significantly improved and extended the use of amplicons, both in cultured cells and in living organisms. In addition, this review also discusses the many difficulties still pending to be solved, in order to achieve stable and physiologically regulated transgene expression.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Herpesvirus Humano 1
/
Técnicas de Transferência de Genes
/
Transgenes
/
Vetores Genéticos
Limite:
Animais
/
Humanos
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
2009
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
França
Instituição/País de afiliação:
Université de Lyon/FR