Free radical scavenging and antimicrobial properties of extracts of wild mushrooms / Atividade sequestradora de radicais livres e propriedades antimicrobianas de extratos de cogumelos selvagens

ABSTRACT

Antioxidant and antimicrobial potentials of extracts obtained from four wild mushrooms, Termitomyces clypeatus (TCE), Termitomyces robustus (TRE), Lentinus subnudus (LSE) and Lenzites species (LZE) collected in Nigeria were investigated. LSE and LZE displayed good scavenging activity against 2, 2-Diphenyl-1-Picrylhydrazyl (DPPH) and ferrous ion radicals at concentration of 2 mg/mL. However, TRE and TCE exhibited better superoxide anion scavenging effect at 2 mg/mL. All extracts (TCE, TRE, LSE and LZE) had comparable scavenging effect on hydroxyl radicals as butylated Hydroxytoluene (BHT) used as control. Moreover, extracts from the wild mushrooms were able to inhibit the growth of all indicator organisms at concentrations between 12.5 mg/mL to 100 mg/mL. LSE and LZE, however, showed better antimicrobial effect on the indicator organisms. The results suggest that extracts obtained from the four wild mushrooms may serve as sources of new bioactive compounds with effective antioxidant and antimicrobial activity.

RESUMO

Foram investigadas as propriedades antioxidantes e antimicrobianas de extratos obtidos de quatro cogumelos selvagens da Nigéria Termitomyces clypeatus (TCE), Termitomyces robustus (TRE), Lentinus subnudus (LSE) and Lenzites species (LZE). LSE e LZE, na concentração de 2 mg/ml, apresentaram boa atividade sequestrante contra 2,2'-difenil-b-picrilhidrazil (DPPHò) e radicais ferrosos. Entretanto, TER e TCE a 2 mg/ml apresentaram melhor efeito sequestrador de anions superóxido .Todos os extratos apresentaram feito semelhante de seqüestro de radicais hidroxila como BHT usado como controle. Além disso, todos os extratos dos cogumelos selvagens, na concentração de 12,5 mg/ml até 100 mg/ml, foram capazes de inibir a multiplicação de todos os microrganismos indicadores testados, mas LSE e LZE apresentaram efeito antimicrobiano mais intenso. Os resultados sugerem que os extratos obtidos dos quatro cogumelos selvagens podem ser fontes de novos compostos bioativos com atividade antimicrobiana e antioxidante.