Comparative analysis of two immunohistochemical methods for antigen retrieval in the optical lobe of the honeybee Apis mellifera: Myosin-v assay
Biol. Res
; 43(1): 7-12, 2010. ilus
Artigo
em Inglês
| LILACS
| ID: lil-548024
Biblioteca responsável:
BR1.1
ABSTRACT
The present study compared two heating methods currently used for antigen retrieval (AR) immunostaining the microwave oven and the steam cooker. Myosin-V, a molecular motor involved in vesicle transport, was used as a neuronal marker in honeybee Apis mellifera brains fixed in formalin. Overall, the steam cooker showed the most satisfactory AR results. At 100 ºC, tissue morphology was maintained and revealed epitope recovery, while evaporation of the AR solution was markedly reduced; this is important for stabilizing the sodium citrate molarity of the AR buffer and reducing background effects. Standardization of heat-mediated AR of formalin-fixed and paraffin-embedded tissue sections results in more reliable immunostaining of the honeybee brain.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Abelhas
/
Imuno-Histoquímica
/
Lobo Óptico de Animais não Mamíferos
/
Miosina Tipo V
/
Antígenos
Limite:
Animais
Idioma:
Inglês
Revista:
Biol. Res
Assunto da revista:
Biologia
Ano de publicação:
2010
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Universidade Federal de Uberlândia/BR
/
Universidade de São Paulo/BR