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Low productivity of ribonucleotide reductase in Saccharomyces cerevisiae increases sensitivity to stannous chloride
Basso, T. S; Pungartnik, C; Brendel, M.
Afiliação
  • Basso, T. S; Universidade Estadual de Santa Cruz. Departamento de Ciências Biológicas. Ilhéus. BR
  • Pungartnik, C; Universidade Estadual de Santa Cruz. Departamento de Ciências Biológicas. Ilhéus. BR
  • Brendel, M; Universidade Estadual de Santa Cruz. Departamento de Ciências Biológicas. Ilhéus. BR
Genet. mol. res. (Online) ; 7(1): 1-6, Jan. 2008. ilus
Artigo em Inglês | LILACS | ID: lil-553764
Biblioteca responsável: BR26.1
ABSTRACT
Ribonucleotide reductase (RNR) of the yeast Saccharomyces cerevisiae is a tetrameric protein complex, consisting of two large and two small subunits. The small subunits Y2 and Y4 form a heterodimer and are encoded by yeast genes RNR2 and RNR4, respectively. Loss of Y4 in yeast mutant rnr4delta can be compensated for by up-regulated expression of Y2, and the formation of a small subunit Y2Y2 homodimer that allows for a partially functional RNR. However, rnr4delta mutants exhibit slower growth than wild-type (WT) cells and are sensitive to many mutagens, amongst them UVC and photo-activated mono- and bi-functional psoralens. Cells of the haploid rnr4delta mutant also show a 3- to 4-fold higher sensitivity to the oxidative stress-inducing chemical stannous chloride than those of the isogenic WT. Both strains acquired increased resistance to SnCl2 with age of culture, i.e., 24-h cultures were more sensitive than cells grown for 2, 3, 4, and 5 days in liquid culture. However, the sensitivity factor of three to four (WT/mutant) did not change significantly. Cultures of the rnr4delta mutant in stationary phase of growth always showed higher frequency of budding cells (budding index around 0.5) than those of the corresponding WT (budding index <0.1), pointing to a delay of mitosis/cytokinesis.
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Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Ribonucleotídeo Redutases / Saccharomyces cerevisiae / Compostos de Estanho / Genes Fúngicos / Mutagênicos Idioma: Inglês Revista: Genet. mol. res. (Online) Assunto da revista: Biologia Molecular / Genética Ano de publicação: 2008 Tipo de documento: Artigo País de afiliação: Brasil Instituição/País de afiliação: Universidade Estadual de Santa Cruz/BR