Increased levels of glutamate in the central nervous system are associated with behavioral symptoms in experimental malaria
Braz. j. med. biol. res
; 43(12): 1173-1177, Dec. 2010. ilus
Article
em En
| LILACS
| ID: lil-569001
Biblioteca responsável:
BR1.1
ABSTRACT
Cerebral malaria (CM) is a severe complication resulting from Plasmodium falciparum infection. This condition has been associated with cognitive, behavioral and motor dysfunctions, seizures and coma. The underlying mechanisms of CM are incompletely understood. Glutamate and other metabolites such as lactate have been implicated in its pathogenesis. In the present study, we investigated the involvement of glutamate in the behavioral symptoms of CM. Seventeen female C57BL/6 mice (20-25 g) aged 6-8 weeks were infected with P. berghei ANKA by the intraperitoneal route using a standardized inoculation of 10(6) parasitized red blood cells suspended in 0.2 mL PBS. Control animals (N = 17) received the same volume of PBS. Behavioral and neurological symptoms were analyzed by the SmithKline/Harwell/Imperial College/Royal Hospital/Phenotype Assessment (SHIRPA) battery. Glutamate release was measured in the cerebral cortex and cerebrospinal fluid of infected and control mice by fluorimetric assay. All functional categories of the SHIRPA battery were significantly altered in the infected mice at 6 days post-infection (dpi) (P ≤ 0.05). In parallel to CM symptoms, we found a significant increase in glutamate levels in the cerebral cortex (mean ± SEM; control 11.62 ± 0.90 nmol/mg protein; infected at 3 dpi 10.36 ± 1.17 nmol/mg protein; infected at 6 dpi 26.65 ± 0.73 nmol/mg protein; with EGTA, control 5.60 ± 1.92 nmol/mg protein; infected at 3 dpi 6.24 ± 1.87 nmol/mg protein; infected at 6 dpi 14.14 ± 0.84 nmol/mg protein) and in the cerebrospinal fluid (control 128 ± 51.23 pmol/mg protein; infected 301.4 ± 22.52 pmol/mg protein) of infected mice (P ≤ 0.05). These findings suggest a role of glutamate in the central nervous system dysfunction found in CM.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Plasmodium berghei
/
Sintomas Comportamentais
/
Córtex Cerebral
/
Líquido Cefalorraquidiano
/
Malária Cerebral
/
Ácido Glutâmico
Tipo de estudo:
Diagnostic_studies
/
Risk_factors_studies
Limite:
Animals
Idioma:
En
Revista:
Braz. j. med. biol. res
Assunto da revista:
BIOLOGIA
/
MEDICINA
Ano de publicação:
2010
Tipo de documento:
Article
País de publicação:
Brasil