Correlation of meta 1 expression with culture stage, cell morphology and infectivity in Leishmania (Leishmania) amazonensis promastigotes
Mem. Inst. Oswaldo Cruz
; 106(2): 190-193, Mar. 2011. graf, tab
Article
em En
| LILACS
| ID: lil-583944
Biblioteca responsável:
BR1.1
ABSTRACT
The parasitic protozoan Leishmania (Leishmania) amazonensis alternates between mammalian and insect hosts. In the insect host, the parasites proliferate as procyclic promastigotes andthen differentiate into metacyclic infective forms. The meta 1 gene is preferentially expressed during metacyclogenesis. Meta 1 expression profile determination along parasite growth curves revealed that the meta 1 mRNA level peaked at the early stationary phase then decreased to an intermediate level. No correlation was observed between meta 1 expression and infectivity. Conversely, infectivity correlated with the increase of apoptotic cells in the late stationary phase.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Leishmania mexicana
/
RNA Mensageiro
/
RNA de Protozoário
/
Genes de Protozoários
/
Perfilação da Expressão Gênica
Limite:
Animals
Idioma:
En
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
MEDICINA TROPICAL
/
PARASITOLOGIA
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Brasil