2D-immunoblotting analysis of Sporothrix schenckii cell wall
Mem. Inst. Oswaldo Cruz
; 106(2): 248-250, Mar. 2011. ilus
Artigo
em Inglês
| LILACS
| ID: lil-583953
Biblioteca responsável:
BR1.1
ABSTRACT
We utilized two-dimensional gel electrophoresis and immunoblotting (2D-immunoblotting) with anti-Sporothrix schenckii antibodies to identify antigenic proteins in cell wall preparations obtained from the mycelial and yeast-like morphologies of the fungus. Results showed that a 70-kDa glycoprotein (Gp70) was the major antigen detected in the cell wall of both morphologies and that a 60-kDa glycoprotein was present only in yeast-like cells. In addition to the Gp70, the wall from filament cells showed four proteins with molecular weights of 48, 55, 66 and 67 kDa, some of which exhibited several isoforms. To our knowledge, this is the first 2D-immunoblotting analysis of the S. schenckii cell wall.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Sporothrix
/
Glicoproteínas de Membrana
/
Parede Celular
/
Antígenos de Fungos
Limite:
Animais
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
2011
Tipo de documento:
Artigo
País de afiliação:
México
Instituição/País de afiliação:
Universidad Juárez del Estado de Durango/MX
/
Universidad Nacional Autónoma de México/MX
/
Universidad de Guanajuato/MX