Morphological and molecular description of Blastocrithidia cyrtomeni sp. nov. (Kinetoplastea: Trypanosomatidae) associated with Cyrtomenus bergi Froeschner (Hemiptera: Cydnidae) from Colombia
Mem. Inst. Oswaldo Cruz
; 106(3): 301-307, May 2011. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-589038
Biblioteca responsável:
BR1.1
ABSTRACT
A new trypanosomatid species, Blastocrithidia cyrtomeni, is herein described using morphological and molecular data. It was found parasitising the alimentary tract of the insect host Cyrtomenus bergi, a polyphagous pest. The morphology of B. cyrtomeni was investigated using light and transmission microscopy and molecular phylogeny was inferred from the sequences of spliced leader RNA (SL rRNA) - 5S rRNA gene repeats and the 18S small subunit (SSU) rRNA gene. Epimastigotes of variable size with straphanger cysts adhering to the middle of the flagellum were observed in the intestinal tract, hemolymph and Malpighian tubules. Kinetoplasts were always observed anterior to the nucleus. The ultrastructure of longitudinal sections of epimastigotes showed the flagellum arising laterally from a relatively shallow flagellar pocket near the kinetoplast. SL RNA and 5S rRNA gene repeats were positive in all cases, producing a 0.8-kb band. The amplicons were 797-803 bp long with > 98.5 percent identity, indicating that they originated from the same organism. According to the sequence analysis of the SL-5S rRNA gene repeats and the 18S SSU rRNA gene, B. cyrtomeni is different from all other known species or isolates of Trypanosomatidae. Both analyses indicate that among known species, it is most closely related to Blastocrithidia triatomae.
Texto completo:
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Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
DNA de Protozoário
/
RNA de Protozoário
/
Trypanosomatina
/
RNA Líder para Processamento
/
Hemípteros
Tipo de estudo:
Fatores de risco
Limite:
Animais
País/Região como assunto:
América do Sul
/
Colômbia
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
2011
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Colômbia
/
Estados Unidos
Instituição/País de afiliação:
International Center of Tropical Agriculture/CO
/
Universidad Nacional de Colombia/CO
/
Universidad del Cauca/CO
/
Universidad del Valle/CO
/
University of California/US