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Bovine parthenogenotes produced by inhibition of first or second polar bodies emission
Bevacqua, Romina J; Fernandez-Martin, Rafael; Salamone, Daniel F.
Afiliação
  • Bevacqua, Romina J; Universidad de Buenos Aires. Facultad de Agronomía. Laboratorio de Biotecnología Animal. Buenos Aires. AR
  • Fernandez-Martin, Rafael; Universidad de Buenos Aires. Facultad de Agronomía. Laboratorio de Biotecnología Animal. Buenos Aires. AR
  • Salamone, Daniel F; Universidad de Buenos Aires. Facultad de Agronomía. Laboratorio de Biotecnología Animal. Buenos Aires. AR
Biocell ; 35(1): 1-7, Apr. 2011. ilus, tab, graf
Artigo em Inglês | LILACS | ID: lil-595004
Biblioteca responsável: AR40.1
ABSTRACT
Parthenogenetic embryos are an ethically acceptable alternative for the derivation of human embryonic stem cells. In this work, we propose a new strategy to produce bovine parthenogenetic embryos inhibiting the emission of the first polar body during in vitro maturation, and allowing the extrusion of the second polar body during oocyte activation. Cytochalasin B, an inhibitor of actin microfilaments, was employed during in vitro maturation to inhibit first polar body emission or during parthenogenetic activation to block second polar body emission. Only one polar body was inhibited in each strategy in order to keep the diploid chromosome set. In experiment 1, the effect of cytochalasin B on in vitro maturation of bovine oocytes was evaluated. Most oocytes (77%) were arrested at a meiotic stage characterized by the presence of a large internal metaphase plate and absence of polar body. In experiment 2, development of embryos exposed to cytochalasin B during in vitro maturation (CytoB-IVM) or during activation (CytoB-ACT) was compared. Developmental rates did not differ between diploidization strategies, even when three agents were employed to induce activation. Both groups, CytoB-IVM and CytoB-ACT, tended to maintain diploidy. CytoB-IVM parthenogenesis could help to obtain embryos with a higher degree of homology to the oocyte donor.
Assuntos
Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Oócitos / Citocalasina B / Embrião de Mamíferos / Meiose Aspecto: Aspectos éticos Limite: Animais / Feminino / Humanos Idioma: Inglês Revista: Biocell Assunto da revista: C‚lulas Ano de publicação: 2011 Tipo de documento: Artigo País de afiliação: Argentina Instituição/País de afiliação: Universidad de Buenos Aires/AR
Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Oócitos / Citocalasina B / Embrião de Mamíferos / Meiose Aspecto: Aspectos éticos Limite: Animais / Feminino / Humanos Idioma: Inglês Revista: Biocell Assunto da revista: C‚lulas Ano de publicação: 2011 Tipo de documento: Artigo País de afiliação: Argentina Instituição/País de afiliação: Universidad de Buenos Aires/AR
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