Production and characterization of PHA from recombinant E. coli harbouring phaC1 gene of indigenous Pseudomonas sp. LDC-5 using molasses
Braz. j. microbiol
; 42(3): 1109-1118, July-Sept. 2011. ilus
Artigo
em Inglês
| LILACS
| ID: lil-607542
Biblioteca responsável:
BR32.1
ABSTRACT
Polyhydroxyalkanoates (PHA) are biodegradable and biocompatible green thermoplastics, synthesized by wide variety of bacteria as an intracellular carbon and energy storage intermediate. They are used as an alternative to nonrenewable petroleum derived plastics. The current interest in these biopolyesters is stimulated by the search for cost-effective capitalized production. This paper attempts to achieve maximized production rate from recombinant system using inexpensive substrate. Molasses from agro-industrial waste was used to produce PHA from recombinant E.coli in batch culture. PHA yield in molasses (3.06g/L ± 0.05-75.5 percent) was higher than that of sucrose (2.5g/L ± 0.05 - 65.1 percent). Properties of the polymer produced from molasses and sucrose were analyzed by DSC, TGA, DTA, GC/MS, TLC and optical rotation studies. The findings suggested that molasses enhanced PHA production in recombinant E.coli.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Polímeros
/
Pseudomonas
/
Sacarose
/
Biopolímeros
/
Melaço
/
Proteínas de Escherichia coli
/
Poli-Hidroxialcanoatos
Idioma:
Inglês
Revista:
Braz. j. microbiol
Assunto da revista:
Microbiologia
Ano de publicação:
2011
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Índia
Instituição/País de afiliação:
Lady Doak College/IN