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Simultaneous detection of hepatitis B virus genotypes and mutations associated with resistance to lamivudine, adefovir, and telbivudine by the polymerase chain reaction-ligase detection reaction
Wang, Yong-Zhong; Xiao, Jun-Hua; Liu, Long-Gen; Ye, Chun-Yan; Shen, Hong-Yu; Xu, Tian-Min; Zhu, Ke-Zhuan.
Afiliação
  • Wang, Yong-Zhong; The Third People's Hospital of Changzhou. Institute for the Study of Liver Diseases. CN
  • Xiao, Jun-Hua; Donghua University. College of Chemistry, Chemical Engineering and Biotechnology. CN
  • Liu, Long-Gen; The Third People's Hospital of Changzhou. Institute for the Study of Liver Diseases. Department of Infectious Diseases. CN
  • Ye, Chun-Yan; The Third People's Hospital of Changzhou. Institute for the Study of Liver Diseases. Department of Infectious Diseases. CN
  • Shen, Hong-Yu; The Third People's Hospital of Changzhou. Institute for the Study of Liver Diseases. CN
  • Xu, Tian-Min; The Third People's Hospital of Changzhou. Institute for the Study of Liver Diseases. Department of Infectious Diseases. CN
  • Zhu, Ke-Zhuan; The Third People's Hospital of Changzhou. Institute for the Study of Liver Diseases. Department of Infectious Diseases. CN
Braz. j. infect. dis ; 15(6): 560-566, Nov.-Dec. 2011. ilus, tab
Artigo em Inglês | LILACS | ID: lil-610527
Biblioteca responsável: BR1.1
ABSTRACT

OBJECTIVES:

Detection of mutations associated to nucleos(t)ide analogs and hepatitis B virus (HBV) genotyping are essential for monitoring treatment of HBV infection. We developed a multiplex polymerase chain reaction-ligase detection reaction (PCR-LDR) assay for the rapid detection of HBV genotypes and mutations associated with lamivudine, adefovir, and telbivudine resistance in HBV-infected patients.

METHODS:

HBV templates were amplified by PCR, followed by LDR and electrophoresis on a sequencer. The assay was evaluated using plasmids that contained wild-type or mutant HBV sequences and 216 clinical samples.

RESULTS:

The PCR-LDR assay and sequencing gave comparable results for 158 of the 216 samples (73.1 percent) with respect to mutation detection and genotyping. Complete agreement between the two methods was observed for all the samples (100 percent) at codon 180 and codon 204. Concordant results were observed for 99.4 percent of the 158 samples at codon 181 and 98.7 percent at codon 236. The genotyping results were completely concordant between the PCR-LDR assay and sequencing. The PCR-LDR assay could detect a proportion of 1 percent mutant plasmid in a background of wild-type plasmid.

CONCLUSION:

The PCR-LDR assay is sensitive and specific for detection of HBV genotypes and drug resistance mutations, and could be helpful for decision making in the treatment of HBV infection.
Assuntos


Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Antivirais / Ácidos Fosforosos / Pirimidinonas / Adenina / Vírus da Hepatite B / Lamivudina / Farmacorresistência Viral Múltipla / Mutação / Nucleosídeos Tipo de estudo: Estudo diagnóstico / Estudo prognóstico / Fatores de risco Limite: Humanos Idioma: Inglês Revista: Braz. j. infect. dis Assunto da revista: Doenças Transmissíveis Ano de publicação: 2011 Tipo de documento: Artigo / Documento de projeto País de afiliação: China Instituição/País de afiliação: Donghua University/CN / The Third People's Hospital of Changzhou/CN

Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Antivirais / Ácidos Fosforosos / Pirimidinonas / Adenina / Vírus da Hepatite B / Lamivudina / Farmacorresistência Viral Múltipla / Mutação / Nucleosídeos Tipo de estudo: Estudo diagnóstico / Estudo prognóstico / Fatores de risco Limite: Humanos Idioma: Inglês Revista: Braz. j. infect. dis Assunto da revista: Doenças Transmissíveis Ano de publicação: 2011 Tipo de documento: Artigo / Documento de projeto País de afiliação: China Instituição/País de afiliação: Donghua University/CN / The Third People's Hospital of Changzhou/CN
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