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Cell alterations induced by a biotherapic for influenza / Cell alterations induced by a biotherapic for influenza
Siqueira, Camila Monteiro; Costa, Beatriz Guerreiro; Ferreira, Ana Maria; Gonçalves, Marta; Veiga, Venicio Feo da; Lyrio, Carlos; Takyia, Christina; Branco, Morgana Castelo; Zancan, Patrícia; Couceiro, José Nelson; Holandino, Carla.
Afiliação
  • Siqueira, Camila Monteiro; Faculdade de Farmácia, Universidade Federal do Rio de Janeiro (UFRJ). RJ. BR
  • Costa, Beatriz Guerreiro; Faculdade de Farmácia, Universidade Federal do Rio de Janeiro (UFRJ). RJ. BR
  • Ferreira, Ana Maria; Instituto de Microbiologia Prof. Paulo de Góes, UFRJ. RJ. BR
  • Gonçalves, Marta; Instituto de Microbiologia Prof. Paulo de Góes, UFRJ. RJ. BR
  • Veiga, Venicio Feo da; Instituto de Microbiologia Prof. Paulo de Góes, UFRJ. RJ. BR
  • Lyrio, Carlos; Instituto Roberto Costa. Petrópolis. BR
  • Takyia, Christina; Laboratório de Patologia Celular, UFRJ. RJ. BR
  • Branco, Morgana Castelo; Faculdade de Farmácia, Universidade Federal do Rio de Janeiro (UFRJ). RJ. BR
  • Zancan, Patrícia; Laboratório de Oncobiologia Molecular, UFRJ. RJ. BR
  • Couceiro, José Nelson; Instituto de Microbiologia Prof. Paulo de Góes, UFRJ. RJ. BR
  • Holandino, Carla; Faculdade de Farmácia, Universidade Federal do Rio de Janeiro (UFRJ). RJ. BR
Artigo em Inglês | LILACS | ID: lil-621613
Biblioteca responsável: BR1.1
ABSTRACT

Introduction:

Influenza viruses have been responsible for highly contagious acute respiratory illnesses with high mortality, mainly in the elderly, which encourages the development of new drugs for the treatment of human flu. The biotherapics are medicines prepared from biological products, which are not chemically defined. They are compounded following the homeopathic procedures indicated for infectious diseases with known etiology [1].

Aim:

The purpose of the present study is to verify cellular alterations induced by a biotherapic prepared from the infectious influenza A virus.

Methodology:

This biotherapic was prepared for this study in the homeopathic potency of 30X according to the Brazilian Homeopathic Pharmacopeia [2]. The concentration of 10% was not cytotoxic to cells, as verified by neutral red assay. The cellular alterations observed in MDCK cells were analyzed by optical microscopy for the quantification of mitosis, nucleoli and lipid bodies. The mitochondrial activity was assessed by MTT assay and the phosphosfructokinase-1 (PFK-1) enzyme activity was analyzed on the MDCK cells treated for 5, 10 and 30 days. Macrophages J778.G8 were treated with this biotherapic to evaluate the immunostimulatory cytokine release.

Results:

The cellular alterations observed in MDCK cells were verified by optical microscopy. The number of lipid bodies present in MDCK cells stimulated for 10 days was significantly lower (p <0.05) when compared to controls. The biotherapic significantly increased (p <0.05) the number of mitosis and the mitochondrial activity of MDCK cells stimulated for 10 and 30 days. These changes were confirmed by a significant reduction (p <0.05) on the PFK-1 activity. These results suggest that the biotherapic was able to activate the Krebs cycle and pentosephosphate metabolism to the generation of amino acids and nucleotides, situations common to cells whose rate of mitosis is increased. The quantification of immunostimulatory cytokines by macrophages J774.G8 indicated that the tumor necrosis factor (TNF-?) production was higher (p <0.05) in the supernatant of the macrophages pre-treated with this biotherapic and infected with influenza virus, suggesting an activation of the macrophages by this biotherapic.

Conclusion:

This biotherapic is able to induce some cellular alterations, which show strong evidence that it might be a promising option for the human flu. New experiments are being developed to understand the mechanisms of action of this biotherapic.

Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Idioma: Inglês Revista: Int. j. high dilution res Assunto da revista: Homeopatia / Terapias Complementares Ano de publicação: 2011 Tipo de documento: Artigo País de afiliação: Brasil Instituição/País de afiliação: Faculdade de Farmácia, Universidade Federal do Rio de Janeiro (UFRJ)/BR / Instituto Roberto Costa/BR / Instituto de Microbiologia Prof. Paulo de Góes, UFRJ/BR / Laboratório de Oncobiologia Molecular, UFRJ/BR / Laboratório de Patologia Celular, UFRJ/BR
Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Idioma: Inglês Revista: Int. j. high dilution res Assunto da revista: Homeopatia / Terapias Complementares Ano de publicação: 2011 Tipo de documento: Artigo País de afiliação: Brasil Instituição/País de afiliação: Faculdade de Farmácia, Universidade Federal do Rio de Janeiro (UFRJ)/BR / Instituto Roberto Costa/BR / Instituto de Microbiologia Prof. Paulo de Góes, UFRJ/BR / Laboratório de Oncobiologia Molecular, UFRJ/BR / Laboratório de Patologia Celular, UFRJ/BR
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