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Effect of Bothrops alternatus snake venom on macrophage phagocytosis and superoxide production: participation of protein kinase C
Setubal, S. S; Pontes, A. S; Furtado, J. L; Kayano, A. M; Stábeli, R. G; Zuliani, J. P.
Afiliação
  • Setubal, S. S; Tropical Pathology Research Institute. Laboratory of Biochemistry and Biotechnology and Laboratory of Cell Culture and Monoclonal Antibodies. BR
  • Pontes, A. S; Tropical Pathology Research Institute. Laboratory of Biochemistry and Biotechnology and Laboratory of Cell Culture and Monoclonal Antibodies. BR
  • Furtado, J. L; Tropical Pathology Research Institute. Laboratory of Biochemistry and Biotechnology and Laboratory of Cell Culture and Monoclonal Antibodies. BR
  • Kayano, A. M; Tropical Pathology Research Institute. Laboratory of Biochemistry and Biotechnology and Laboratory of Cell Culture and Monoclonal Antibodies. BR
  • Stábeli, R. G; Tropical Pathology Research Institute. Laboratory of Biochemistry and Biotechnology and Laboratory of Cell Culture and Monoclonal Antibodies. BR
  • Zuliani, J. P; Tropical Pathology Research Institute. Laboratory of Biochemistry and Biotechnology and Laboratory of Cell Culture and Monoclonal Antibodies. BR
J. venom. anim. toxins incl. trop. dis ; 17(4): 430-441, 2011. graf
Artigo em Inglês | LILACS | ID: lil-623506
Biblioteca responsável: BR33.1
ABSTRACT
Envenomations caused by different species of Bothrops snakes result in severe local tissue damage, hemorrhage, pain, myonecrosis, and inflammation with a significant leukocyte accumulation at the bite site. However, the activation state of leukocytes is still unclear. According to clinical cases and experimental work, the local effects observed in envenenomation by Bothrops alternatus are mainly the appearance of edema, hemorrhage, and necrosis. In this study we investigated the ability of Bothrops alternatus crude venom to induce macrophage activation. At 6 to 100 »g/mL, BaV is not toxic to thioglycollate-elicited macrophages; at 3 and 6 »g/mL, it did not interfere in macrophage adhesion or detachment. Moreover, at concentrations of 1.5, 3, and 6 »g/mL the venom induced an increase in phagocytosis via complement receptor one hour after incubation. Pharmacological treatment of thioglycollate-elicited macrophages with staurosporine, a protein kinase (PKC) inhibitor, abolished phagocytosis, suggesting that PKC may be involved in the increase of serum-opsonized zymosan phagocytosis induced by BaV. Moreover, BaV also induced the production of anion superoxide (O2-) by thioglycollate-elicited macrophages. This BaV stimulated superoxide production was abolished after treating the cells with staurosporine, indicating that PKC is an important signaling pathway for the production of this radical. Based on these results, we suggest that phagocytosis and reactive oxygen species are involved in the pathogenesis of local tissue damage characteristic of Bothrops spp. envenomations.
Assuntos


Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Fagocitose / Proteínas Quinases / Bothrops / Venenos de Crotalídeos / Macrófagos / Animais Venenosos Limite: Animais Idioma: Inglês Revista: J. venom. anim. toxins incl. trop. dis Assunto da revista: Toxicologia Ano de publicação: 2011 Tipo de documento: Artigo País de afiliação: Brasil Instituição/País de afiliação: Tropical Pathology Research Institute/BR

Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Fagocitose / Proteínas Quinases / Bothrops / Venenos de Crotalídeos / Macrófagos / Animais Venenosos Limite: Animais Idioma: Inglês Revista: J. venom. anim. toxins incl. trop. dis Assunto da revista: Toxicologia Ano de publicação: 2011 Tipo de documento: Artigo País de afiliação: Brasil Instituição/País de afiliação: Tropical Pathology Research Institute/BR
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