Identification of chilling-responsive transcripts in peanut (Arachis hypogaea L. )
Electron. j. biotechnol
; 14(5): 5-5, Sept. 2011. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-640512
Biblioteca responsável:
CL1.1
ABSTRACT
To isolate differentially expressed peanut genes responsive to chilling, a suppression subtractive hybridization (SSH) cDNA library was constructed for a chilling tolerant peanut cultivar A4 with mRNAs extracted from the seeds imbibed at 2ºC and 15ºC, respectively, for 24 hrs. A total of 466 cDNA clones were sequenced, from which 193 unique transcripts (73 contigs and 120 singlets) were assembled. Of these unique transcripts, 132 (68.4 percent) were significantly similar to the sequences in GenBank non-redundant (nr) protein database, which belonged to diverse functional categories including metabolism, signal transduction, stress response, cell defense and transcriptional regulation. The remaining 61 (31.6 percent) showed no similarity to either hypothetical or known proteins. Six differentially expressed transcripts were further confirmed with real-time quantitative PCR (RT-qPCR).
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Arachis
/
Reação em Cadeia da Polimerase
/
Temperatura Baixa
/
Hibridização de Ácido Nucleico
Tipo de estudo:
Estudo diagnóstico
Idioma:
Inglês
Revista:
Electron. j. biotechnol
Assunto da revista:
Biotecnologia
Ano de publicação:
2011
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
China
Instituição/País de afiliação:
Jilin Academy of Agricultural Sciences/CN
/
Ocean University of China/CN
/
Shandong Peanut Research Institute/CN
/
Zhengzhou Tobacco Research Institute of CNTC/CN