Construction of an engineered alpha 1-antitrypsin with inhibitory activity based on theoretical studies
Electron. j. biotechnol
; 15(2): 8-8, Mar. 2012. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-640543
Biblioteca responsável:
CL1.1
ABSTRACT
Background:
The elastase inhibitor alpha-1-antitrypsin (AAT), is a member of the serpin superfamily of protease inhibitors. AAT has a characteristic secondary structure of three-beta-sheets, nine-alpha-helices and a reactive central loop (RCL). This protein inhibits target proteases by forming a stable complex in which the cleaved RCL is inserted into beta-sheet-A of the serpin, leading to a conformational change in the AAT protein. Spontaneous polymerization and instability of AAT are challenges with regard to producing drugs against AAT-deficient diseases. Therefore, the purpose of many investigations currently is to produce drugs with lower degrees of polymerization and higher stabilities. In order to investigate the effect of the N-terminal segment (residues 1-43) on AAT structure, molecular dynamic (MD) simulation was used to study structural properties including Root-mean-square deviation (RMSD), internal motions, intramolecular non-bonded interactions and the total accessible surface area (ASA) of native and reduced AAT. These properties were compared in native and truncated AAT.Results:
Theoretical studies showed no noticeable differences in the dynamic and structural properties of the two structures. These findings provided the basis for the experimental phase of the study in which sequences from the two AAT constructs were inserted into the expression vector pGAPZ and transformed into Pichia pastoris. Results showed no differences in the activities and polymerization of the two AAT constructs.Conclusions:
As small-scale medicines are preferred by lung drug delivery systems, in this study AAT was designed and constructed by decreasing the number of amino acids at the N-terminal region.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Contexto em Saúde:
Doenças Negligenciadas
Problema de saúde:
Hanseníase
Base de dados:
LILACS
Assunto principal:
Pichia
/
Inibidores da Tripsina
/
Alfa 1-Antitripsina
/
Simulação de Dinâmica Molecular
Limite:
Humanos
Idioma:
Inglês
Revista:
Electron. j. biotechnol
Assunto da revista:
Biotecnologia
Ano de publicação:
2012
Tipo de documento:
Artigo
País de afiliação:
Irã
Instituição/País de afiliação:
Tarbiat Modares University/IR
/
University of Guilan/IR